低氧誘導因子2α(HIF2a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Hypoxia Inducible Factor 2 Alpha (HIF2a)
EPAS1; HLF; MOP2; PASD2; BHLHE73; Endothelial PAS Domain 1; HIF-1 Alpha-Like Factor; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73
- 編號KSD466Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍78-5000pg/mL
- 可適靈敏度29pg/mL
- 應用Main materials for "Do It(ELISA Kit) Yourself"
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 8208 ¥ 13680 ¥ 24624 ¥ 47880 ¥ 82080
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特異性
試劑盒中的抗體對低氧誘導因子2α(HIF2a)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPD466Ra01 | 低氧誘導因子2α(HIF2a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAD466Ra01 | 低氧誘導因子2α(HIF2a)多克隆抗體 | WB; IHC; ICC; IP. |
LAD466Ra71 | 低氧誘導因子2α(HIF2a)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAD466Ra21 | 低氧誘導因子2α(HIF2a)單克隆抗體 | WB; IHC; ICC; IP. |
SED466Ra | 低氧誘導因子2α(HIF2a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMD466Ra | 低氧誘導因子2α(HIF2a)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSD466Ra01 | 低氧誘導因子2α(HIF2a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻
雜志 | 參考文獻 |
Neuro Oncol | Combination viroimmunotherapy with checkpoint inhibition to treat glioma, based on location-specific tumor profiling [PubMed: 26409567] |
Clinical Trials in Degenerative Diseases | Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial [issn:2542-3975] |
Journal of Physiological Anthropology | Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia [Pubmed:29454386] |
Oxidative damage and iron dyshomeostasis in thalassemic patients [] | |
Neuropsychiatric Disease and Treatment | The Association Between Hypoxia Improvement and Electroconvulsive Therapy for Major Depressive Disorder [34588778] |
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