白介素17F(IL17F)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Interleukin 17F (IL17F)
ML-1
- 編號KSB955Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍7.8-500pg/mL
- 可適靈敏度2.7pg/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
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特異性
試劑盒中的抗體對白介素17F(IL17F)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
APB955Hu61 | 白介素17F(IL17F)活性蛋白 | Cell?culture;?Activity?Assays. |
EPB955Hu61 | 白介素17F(IL17F)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB955Hu01 | 白介素17F(IL17F)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB955Hu01 | 白介素17F(IL17F)活性蛋白 | Cell?culture;?Activity?Assays. |
PAB955Hu01 | 白介素17F(IL17F)多克隆抗體 | WB; IHC; ICC; IP. |
LAB955Hu81 | 白介素17F(IL17F)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
LAB955Hu71 | 白介素17F(IL17F)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAB955Hu22 | 白介素17F(IL17F)單克隆抗體 | WB; IHC; ICC; IP. |
MAB955Hu23 | 白介素17F(IL17F)單克隆抗體 | WB; IHC; ICC; IP. |
MAB955Hu21 | 白介素17F(IL17F)單克隆抗體 | WB. |
SEB955Hu | 白介素17F(IL17F)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB955Hu | 白介素17F(IL17F)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB955Hu | 白介素17F(IL17F)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB955Hu01 | 白介素17F(IL17F)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
Cytokine | Expression of IL-17A and IL-17F in lipopolysaccharide-induced acute lung injury and the counteraction of anisodamine or methylprednisolone [ScienceDirect: S1043466614000040] |
Medical Sciences | Cerebrospinal Fluid (CSF) IL-17A, IL-17F, IL-34 and CXCL-13 Levels in Amyotrophic Lateral Sclerosis (ALS/MND) Patients [] |
Revista da Associacao Medica Brasileira | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?… [] |
Hum Exp Toxicol | Chrysene accelerates the proceeding of chronic obstructive pulmonary disease with the aggravation of inflammation and apoptosis in cigarette smoke exposed mice [33345606] |
Rev Assoc Med Bras | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic??- [33295414] |
J Periodontol | Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study [34730850] |
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