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甘露糖結(jié)合蛋白(MBL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Mannose Binding Lectin (MBL)

MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

  • 甘露糖結(jié)合蛋白(MBL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 甘露糖結(jié)合蛋白(MBL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)甘露糖結(jié)合蛋白(MBL)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。

配套試劑

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編號(hào) 適用物種:Chicken (Gallus,雞) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB480Ga01 甘露糖結(jié)合蛋白(MBL)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB480Ga01 甘露糖結(jié)合蛋白(MBL)多克隆抗體 WB
MAB480Ga21 甘露糖結(jié)合蛋白(MBL)單克隆抗體 WB; IHC; ICC; IP.
SEB480Ga 甘露糖結(jié)合蛋白(MBL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB480Ga 甘露糖結(jié)合蛋白(MBL)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB480Ga01 甘露糖結(jié)合蛋白(MBL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
American Journal of Nephrology Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. [Karger: 000255439]
Current Eye Research Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle Glaucoma [Ingenta: art00007]
Journal of Clinical Immunology Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511]
Clinical and Experimental Immunology Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathy [PubMed: PMC3406374]
Clin J Am Soc Nephrol. Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GN [Pubmed: 24115193]
ACTA TROPICA Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis [Pubmed: 32827456]
PLoS One Complement activation profile of patients with primary focal segmental glomerulosclerosis [Pubmed: 32569286]
LIFE SCIENCES The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes [34242658]
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