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胃動素(MTL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

試劑盒中的抗體對胃動素(MTL)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

雜志	參考文獻
世界華人消化雜志	清醒狀態(tài)下熱射病大鼠胃腸動力與胃泌素、胃動素的變化 [Wjgnet:Source]
Sci Rep.	Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut. [pubmed:28054652]
Applied Biological Chemistry	Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation [10.1007/s13765-017-0327-3]
Biomed Research International	Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation [pubmed:29082242]
Evidence-Based?Complementary and Alternative Medicine	Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids [Pubmed:29853946]
The American Journal of the?Medical?Sciences	The Relations Among Serum Ghrelin, Motilin and Gastric Emptying and Autonomic Function in Autoimmune Gastritis [Pubmed:29753372]
NEUROGASTROENTEROLOGY AND MOTILITY	Integrative effects of transcutaneous electrical acustimulation on abdominal pain, gastrointestinal motility, and inflammation in patients with early‐stage acute?… [34536258]
Evid Based Complement Alternat Med	The Inhibitory Effects of Naringin in a Rat Model of Postoperative Intraperitoneal Adhesion Formation [Pubmed:35069760]

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編號	適用物種：Rattus norvegicus (Rat，大鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA575Ra01	胃動素(MTL)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAA575Ra01	胃動素(MTL)多克隆抗體	WB; IHC; ICC; IP.
MAA575Ra21	胃動素(MTL)單克隆抗體	WB; IHC; ICC; IP.
CEA575Ra	胃動素(MTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA575Ra	胃動素(MTL)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSA575Ra11	胃動素(MTL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".