肌肉生長抑制素(MSTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Myostatin (MSTN)
GDF8; Growth Differentiation Factor 8
- 編號KSB653Ra11
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍1.11-90ng/mL
- 可適靈敏度0.44ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對肌肉生長抑制素(MSTN)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB653Ra01 | 肌肉生長抑制素(MSTN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB653Ra01 | 肌肉生長抑制素(MSTN)活性蛋白 | Cell?culture;?Activity?Assays. |
CPB653Ra21 | 肌肉生長抑制素(MSTN)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAB653Ra08 | 肌肉生長抑制素(MSTN)多克隆抗體 | WB; IHC; ICC; IP. |
PAB653Ra01 | 肌肉生長抑制素(MSTN)多克隆抗體 | WB,IHC |
LAB653Ra71 | 肌肉生長抑制素(MSTN)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAB653Ra21 | 肌肉生長抑制素(MSTN)單克隆抗體 | WB; IHC; ICC; IP. |
SEB653Ra | 肌肉生長抑制素(MSTN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEB653Ra | 肌肉生長抑制素(MSTN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB653Ra | 肌肉生長抑制素(MSTN)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB653Ra | 肌肉生長抑制素(MSTN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB653Ra11 | 肌肉生長抑制素(MSTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Clinical Medicine and Diagnostics | Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats [Sapub:Source] |
Nutr Metab (Lond) | Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice [PubMed: 26539241] |
International Journal of Cancer | Tumor inoculation site affects the development of cancer cachexia and muscle wasting [PubMed: 26016447] |
Connect Tissue Res | Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts [PubMed: 25748814] |
PLoS One | Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease [Pubmed:26998756] |
Calcif Tissue Int. | The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats [Pubmed:27272029] |
Archives of medical research | Irisin and Myostatin Levels in Patients with Graves' Disease. [pubmed:27986127] |
Calcified Tissue International | Effect of?Strontium Ranelate on?the?Muscle and?Vertebrae of?Ovariectomized Rats [pubmed:29242963] |
Scientific Reports | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. [pubmed:28740214] |
Journal of?Animal?Science | Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs [Pubmed: 30203098] |
European Journal of Internal Medicine | Predictive value of serum myostatin for the severity and clinical outcome of heart failure [Pubmed: 31056368] |
J Cell Physiol | Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats [Pubmed: 31960436] |
NEPHROLOGY DIALYSIS TRANSPLANTATION | Myostatin and muscle atrophy during chronic kidney disease [Pubmed: 32974666] |
Oxid Med Cell Longev | Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance?… [34868459] |
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