核因子κB(NFkB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Nuclear Factor Kappa B (NFkB)
NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit
- 編號KSB824Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.057ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 6221 ¥ 10368 ¥ 18662 ¥ 36288 ¥ 62208
- 欲了解實(shí)際交易價(jià)格和更多情況,請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
特異性
試劑盒中的抗體對核因子κB(NFkB)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB824Mu01 | 核因子κB(NFkB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB824Mu01 | 核因子κB(NFkB)多克隆抗體 | WB; IHC; ICC; IP. |
LAB824Mu81 | 核因子κB(NFkB)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAB824Mu71 | 核因子κB(NFkB)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MEB824Mu | 核因子κB(NFkB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEB824Mu | 核因子κB(NFkB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB824Mu | 核因子κB(NFkB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB824Mu01 | 核因子κB(NFkB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Reproductice Science | Surgical Removal of Endometrioma Decreases the NF-kB1 (p50/105) and NF-kB p65 (Rel A) Expression in the Eutopic Endometrium During the Implantation Window [Sagepub: Source] |
International Immunopharmacology | Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with TNBS-induced recurrent ulcerative colitis [PubMed: 21642017] |
PLoS One | New Method to Isolate and Culture Rat Kupffer Cells [PubMed: PMC3743898] |
World Journal of Gastroenterology | Long-term aspirin pretreatment in the prevention of cerulein-induced acute pancreatitis in rats [PubMed: PMC3660814] |
Experimental and Therapeutic Medicine | Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB [PubMed: PMC3570159] |
PLoS One. | Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434] |
Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835] |
BBA Clin | Long-term warfarin therapy and biomarkers for osteoporosis and atherosclerosis [PubMed: 26674156] |
J Clin Diagn Res | Serological Analysis of Alpha-synuclein and NF-κB in Parkinson’s Disease Patients [PubMed: 26155470] |
Eur J Obstet Gynecol Reprod Biol | Hysteroscopic polypectomy decreases NF-κB1 expression in the mid-secretory endometrium of women with endometrial polyp [PubMed: 25898371] |
Asian Pacific Journal of Cancer Prevention | Plasma nuclear factor kappa B and serum peroxiredoxin 3 in early diagnosis of hepatocellular carcinoma. [PubMed: 25743848] |
Acta Biochim Pol | Preventive use of Lactobacillus plantarum LS/07 and inulin to relieve symptoms of acute colitis. [PubMed: 26345092] |
Acta Veterinaria | Use Of The Prebiotic Inulin In The Prevention Of Adverse Signs Of Acute Colitis [View: J] |
Neurochem Res | Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat [PubMed: 26700436] |
Transplant Proc | L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients [PubMed: 26518955] |
Journal of Food Science? | Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine [Pubmed:27061742] |
IJBPAS | THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATS [publication:298838934] |
Basic & Clinical Pharmacology & Toxicology | Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathway [doi:10.1111] |
Peptides. | Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. [pubmed:27777064] |
british journal of pharmacology | Forskolin, a hedgehog signalling inhibitor, attenuates carbon tetrachloride-induced liver fibrosis in rats. [pubmed:27590029] |
european journal of pharmacology | Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. [pubmed:27915041] |
european journal of pharmacology | S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. [pubmed:27887948] |
Psychopharmacology (Berl) | Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms. [pubmed:28303372] |
biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883] |
biomedicine & pharmacotherapy | Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation. [pubmed:28033582] |
Toxicology and Applied Pharmacology | Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) [pubmed:28974454] |
Microbial Immunology | Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota [pubmed:28713364] |
Journal of Biomechanics | The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialis [pubmed:29137729] |
European Journal of Pharmacology | In vivo cellular and molecular gastroprotective mechanisms of chrysin; Emphasis on oxidative stress, inflammation and angiogenesis [pubmed:29126792] |
Frontiers in Pharmacology | Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus. [pubmed:28553224] |
International Journal of Medicinal Mushrooms | Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus. [pubmed:29256845] |
Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403] |
International Immunopharmacology | Scutellarin alleviates lipopolysaccharide-induced cognitive deficits in the rat: Insights into underlying mechanisms. [pubmed:29190543] |
Food and Chemical Toxicology | Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088] |
Neurotoxicity Research | Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells [Pubmed:29869772] |
European?Journal of Pharmacology? | Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat [Pubmed:29518393] |
Experimental?biology?and?medicine?(Maywood) | Shock waves increase pulmonary vascular leakage, inflammation, oxidative stress, and apoptosis in a mouse model [Pubmed:29984607] |
Acta?Veterinaria?Belgrade | Anti-inflammatory potential of Lactobacillus plantarum LS/07 in acute colitis in rats [10.2478:acve-2018-0005] |
Taiwanese Journal of Obstetrics & Gynecology | HMGB1-RAGE signaling pathway in pPROM [Pubmed:29673663] |
Acta Veterinaria Brno | Improvement of colitis by LS/07 and inulin [10.2754:avb201786040399] |
Drug?Discoveries & Therapeutics | The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4 [Pubmed:29760339] |
Life?Sciences | Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096] |
Life?Sciences | Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent manner [Pubmed: 30195035] |
Journal?of?Traditional?Chinese?Medical?Sciences | Effect of Dachaihu Decoction on non-alcoholic fatty liver disease model rats induced by a high-fat high-sugar diet [] |
Naunyn-Schmiedeberg's?archives of pharmacology | Amitriptyline attenuates bleomycin-induced pulmonary fibrosis: modulation of the expression of NF-κβ, iNOS, and Nrf2 [Pubmed: 30474696] |
Archives of Physiology and Biochemistry | Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216] |
Journal of Functional Foods | Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments [] |
Scientific Reports | Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis [Pubmed: 30651572] |
Journal of?Pain?Researc | Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain [Pubmed: 30881100] |
Antioxidants | Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian?… [Pubmed: 31600920] |
journal of biochemical and molecular toxicology | Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells [Pubmed: 31916655] |
Life Sci | New insights into the protection of growth hormone in cisplatin-induced nephrotoxicity: The impact of IGF-1 on the Keap1-Nrf2/HO-1 signaling [Pubmed: 32209424] |
European Review for Medical and Pharmacological Sciences | Effects of lncRNA gm4419 on rats with hypertensive cerebral atherosclerosis through NF-κB pathway [Pubmed: 31858567] |
J Ethnopharmacol | Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrhea [Pubmed: 33217518] |
Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |
LIFE SCIENCES | Effects of combined treatment with Indomethacin and Juglone on AOM/DSS induced colon carcinogenesis in Balb/c mice: Roles of inflammation and?… [Pubmed: 33148421] |
JOURNAL OF ETHNOPHARMACOLOGY | Morroniside attenuates apoptosis and pyroptosis of chondrocytes and ameliorates osteoarthritic development by inhibiting NF-κB signaling [Pubmed: 33022338] |
Journal of Cosmetic Dermatology | Examination of androgenetic alopecia with serum biomarkers [Pubmed: 32969583] |
NEUROPSYCHOBIOLOGY | Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms [Pubmed: 32526752] |
journal of ethnopharmacology | UPLC-Orbitrap HRMS metabolic profiling of Cymbopogon citratus cultivated in Egypt; neuroprotective effect against AlCl3-induced neurotoxicity in rats [Pubmed: 32376365] |
BIOMEDICINE & PHARMACOTHERAPY | Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939] |
Journal of Diabetes Research | Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes Mellitus [Pubmed: 33123597] |
Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats [] |
TOXICOLOGY IN VITRO | Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells [33675893] |
Hum Exp Toxicol | Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats [33882726] |
J Food Biochem | Masseter muscle and gingival tissue inflammatory response following treatment with high?\fructose corn syrup in rats: Anti?\inflammatory and antioxidant effects of kefir [33864286] |
Open Access Macedonian Journal of Medical Sciences | The Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway Regulation [] |
Eur Rev Med Pharmacol Sci | Vitamin D supplementation inhibits NF-k? signaling pathway in lean and obese women with PCOS [Pubmed:35731068] |
BMC Nephrol | Effect of subacute poisoning with lambdacyhalothrin on vascular endothelial growth factor 2 receptor in mice kidneys [Pubmed:35643467] |
Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?… [Pubmed:35204140] |
Journal of Pharmaceutical Research International | Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid?… [] |
留言咨詢