YY肽(PYY)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Peptide YY (PYY)
PYY1; Peptide tyrosine tyrosine
- 編號(hào)KSB067Ra11
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍12.35-1000pg/mL
- 可適靈敏度4.71pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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特異性
試劑盒中的抗體對(duì)YY肽(PYY)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB067Ra01 | YY肽(PYY)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPB067Ra21 | YY肽(PYY)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAB067Ra01 | YY肽(PYY)多克隆抗體 | WB; IHC; ICC; IP. |
MAB067Ra21 | YY肽(PYY)單克隆抗體 | WB; IHC; ICC; IP. |
CEB067Ra | YY肽(PYY)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEB067Ra | YY肽(PYY)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB067Ra | YY肽(PYY)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB067Ra11 | YY肽(PYY)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
International Journal of Sport Studies | The Effects of Concurrent Resistance and Endurance Exercise on Hunger Feelings and PYY in Obese Men [Com:Source] |
Annals of Applied Sport Science | Effect of an Acute Incremental Exercise on Plasma Peptide YY, Neuropeptide Y and IGF-1 Concentrations in Young Athletes [Aassjournal:Source] |
Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents. [pubmed:28413639] |
Acta Diabetologica | Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes [pubmed:28620678] |
Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents [10.3892/br.2017.865] |
Journal of Diabetes & Metabolism | High Fiber Fat and Protein Contents Lead to Increased Satiety Reduced Sweet Cravings and Decreased Gastrointestinal Symptoms Independently of Anthropometric Hormonal and Metabolic Factors [10.4172/2155-6156.1000733?] |
Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555] |
Food?and Nutrition?Research | Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet [Pubmed:29599686] |
The Journal of Nutrition | Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811] |
The Journal of Nutritional Biochemistry | Bile acid receptor TGR5 is critically involved in preference for dietary lipids and obesity [Pubmed: 31812910] |
Arch Toxicol | Serotonin involvement in okadaic acid-induced diarrhoea in vivo [34148100] |
foods | Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with?… [34828792] |
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