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血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Platelet Derived Growth Factor Subunit A (PDGFA)

PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide

  • 血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對血小板衍生生長因子A(PDGFA)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA528Hu01 血小板衍生生長因子A(PDGFA)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA528Hu01 血小板衍生生長因子A(PDGFA)活性蛋白 Cell?culture;?Activity?Assays.
LAA528Hu71 血小板衍生生長因子A(PDGFA)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
LAA528Hu81 血小板衍生生長因子A(PDGFA)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
MAA528Hu22 血小板衍生生長因子A(PDGFA)單克隆抗體 WB; IHC; ICC; IP.
MAA528Hu21 血小板衍生生長因子A(PDGFA)單克隆抗體 WB; IHC; ICC; IP.
MAA528Hu23 血小板衍生生長因子A(PDGFA)單克隆抗體 WB; IHC; ICC; IP.
SEA528Hu 血小板衍生生長因子A(PDGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA528Hu 血小板衍生生長因子A(PDGFA)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA528Hu01 血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志 參考文獻
Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680]
International Immunopharmacology Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway [pubmed:28521243]
Open Access Macedonian Journal of Medical Sciences Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells []
Prostaglandins & other Lipid Mediators Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathway [Pubmed: 31698141]
Ann Agric Environ Med PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis [Pubmed: 32208584]
Tissue Eng Regen Med Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method [34339025]
Biomed Research International Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form [Pubmed:35586817]
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