血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Platelet Derived Growth Factor Subunit A (PDGFA)
PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide
- 編號KSA528Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.056ng/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解實際交易價格和更多情況,請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
特異性
試劑盒中的抗體對血小板衍生生長因子A(PDGFA)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA528Hu01 | 血小板衍生生長因子A(PDGFA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA528Hu01 | 血小板衍生生長因子A(PDGFA)活性蛋白 | Cell?culture;?Activity?Assays. |
LAA528Hu71 | 血小板衍生生長因子A(PDGFA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
LAA528Hu81 | 血小板衍生生長因子A(PDGFA)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
MAA528Hu22 | 血小板衍生生長因子A(PDGFA)單克隆抗體 | WB; IHC; ICC; IP. |
MAA528Hu21 | 血小板衍生生長因子A(PDGFA)單克隆抗體 | WB; IHC; ICC; IP. |
MAA528Hu23 | 血小板衍生生長因子A(PDGFA)單克隆抗體 | WB; IHC; ICC; IP. |
SEA528Hu | 血小板衍生生長因子A(PDGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA528Hu | 血小板衍生生長因子A(PDGFA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA528Hu01 | 血小板衍生生長因子A(PDGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
Canadian Journal of Physiology and Pharmacology | The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680] |
International Immunopharmacology | Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway [pubmed:28521243] |
Open Access Macedonian Journal of Medical Sciences | Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells [] |
Prostaglandins & other Lipid Mediators | Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathway [Pubmed: 31698141] |
Ann Agric Environ Med | PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis [Pubmed: 32208584] |
Tissue Eng Regen Med | Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method [34339025] |
Biomed Research International | Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form [Pubmed:35586817] |
留言咨詢