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蛋白激酶Cβ1(PKCb1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Protein Kinase C Beta 1 (PKCb1)

PKC-B1; PRKCB; PRKCB1

  • 蛋白激酶Cβ1(PKCb1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 蛋白激酶Cβ1(PKCb1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對蛋白激酶Cβ1(PKCb1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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KSB045Rb01 蛋白激酶Cβ1(PKCb1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志 參考文獻
Vascular Pharmacology Hesperetin ameliorates hyperglycemia induced retinal vasculopathy via anti-angiogenic effects in experimental diabetic rats [ScienceDirect: S1537189112000407]
Food and Chemical Toxicology Prevention and management of diabetic retinopathy in STZ diabetic rats by Tinospora cordifolia and its molecular mechanisms [PubMed: S0278691512004103]
Journal of ocular pharmacology and therapeutics Retinoprotective Effects of Moringa oleifera via Antioxidant, Anti-Inflammatory, and Anti-Angiogenic Mechanisms in Streptozotocin-Induced Diabetic Rats [Pubmed: 23215831]
Mol Cell Biochem. Effects of Trigonella foenum-graecum (L.) on retinal oxidative stress, and proinflammatory and angiogenic molecular biomarkers in streptozotocin-induced diabetic rats [Pubmed:24242137]
J Clin Diagn Res. Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantia [pmc:PMC4866090]
Journal of Clinical&Diagnostic Research Evaluation?of?Protein?Kinase?Cβ and PPARγ?Activity?in?Diabetic?RatsSupplemented?with?Momordica?charantia. [pubmed:27190792]
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