絲甘蛋白聚糖(SRGN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Serglycin (SRGN)
PRG; PPG; PRG1; proteoglycan 1, secretory granule
- 編號KSC869Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.059ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7110 ¥ 11850 ¥ 21330 ¥ 41475 ¥ 71100
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特異性
試劑盒中的抗體對絲甘蛋白聚糖(SRGN)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPC869Mu01 | 絲甘蛋白聚糖(SRGN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC869Mu01 | 絲甘蛋白聚糖(SRGN)多克隆抗體 | WB; IHC; ICC; IP. |
LAC869Mu81 | 絲甘蛋白聚糖(SRGN)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAC869Mu71 | 絲甘蛋白聚糖(SRGN)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
SEC869Mu | 絲甘蛋白聚糖(SRGN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC869Mu | 絲甘蛋白聚糖(SRGN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSC869Mu01 | 絲甘蛋白聚糖(SRGN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Irish Journal of?Medical?Science | The role of increased synovial fluid A disintegrin and metalloproteinase with thrombospondin motifs4 and serglycin levels in osteoarthritis [Pubmed: 30536194] |
MOLECULAR AND CELLULAR BIOCHEMISTRY | SRGN, a new identified shear-stress-responsive gene in endothelial cells [Pubmed: 32712749] |
Theranostics | Significance of serglycin and its binding partners in autocrine promotion of metastasis in esophageal cancer [33456569] |
CELLULAR ONCOLOGY | Cancer?\associated fibroblasts secrete hypoxia?\induced serglycin to promote head and neck squamous cell carcinoma tumor cell growth in vitro and in vivo by ?- [33651283] |
Cell Death Dis | Serglycin induces osteoclastogenesis and promotes tumor growth in giant cell tumor of bone [34556636] |
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