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存活素(Surv)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Survivin (Surv)

BIRC5; API4; EPR-1; Baculoviral Inhibitor Of Apoptosis Repeat Containing 5; Apoptosis Inhibitor 4; Survivin Variant 3 Alpha

  • 存活素(Surv)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 存活素(Surv)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對存活素(Surv)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPC045Mu01 存活素(Surv)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC045Mu02 存活素(Surv)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC045Mu02 存活素(Surv)多克隆抗體 WB; IHC; ICC; IP.
PAC045Mu01 存活素(Surv)多克隆抗體 WB; IHC; ICC; IP.
SEC045Mu 存活素(Surv)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC045Mu 存活素(Surv)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSC045Mu01 存活素(Surv)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Prion. Heligmosomoides polygyrus antigens inhibit the intrinsic pathway of apoptosis by overexpression of survivin and Bcl-2 protein in CD4 T cells [Pubmed: 23787700]
Medical Oncology Expression of serum survivin protein in diagnosis and prognosis of gallbladder cancer: a comparative study [Pubmed:25129311]
J Surg Res. Prognostic significance of survivin in resected gallbladder cancer [Pubmed:25472573]
Arch. Immunol. Ther. Exp. The Effects of Intestinal Nematode L4 Stage on Mouse Experimental Autoimmune Encephalomyelitis [pubmed:28975357]
International Journal of?Nanomedicine Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor?… [Pubmed:29719390]
Cancers (Basel) In Vivo and In Vitro Enhanced Tumoricidal Effects of Metformin, Active Vitamin D3, and 5-Fluorouracil Triple Therapy against Colon Cancer by Modulating the?… [Pubmed:35326689]
Metformin and Calcitriol Enhance 5-Fluorouracil Tumoricidal Effects in Colon Cancer By Modulating The PI3K/Akt/PTEN/mTOR Network []
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