促甲狀腺素釋放激素(TRH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Thyrotropin Releasing Hormone (TRH)
TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
- 編號KSA839Mu11
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍6.17-500pg/mL
- 可適靈敏度2.39pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對促甲狀腺素釋放激素(TRH)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA839Mu01 | 促甲狀腺素釋放激素(TRH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA839Mu02 | 促甲狀腺素釋放激素(TRH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA839Mu21 | 促甲狀腺素釋放激素(TRH)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA839Mu31 | 促甲狀腺素釋放激素(TRH)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA839Mu08 | 促甲狀腺素釋放激素(TRH)多克隆抗體 | WB; IHC; ICC; IP. |
PAA839Mu01 | 促甲狀腺素釋放激素(TRH)多克隆抗體 | WB; IHC; ICC; IP. |
PAA839Mu02 | 促甲狀腺素釋放激素(TRH)多克隆抗體 | WB; IHC; ICC; IP. |
LAA839Mu81 | 促甲狀腺素釋放激素(TRH)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
CEA839Mu | 促甲狀腺素釋放激素(TRH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA839Mu | 促甲狀腺素釋放激素(TRH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA839Mu11 | 促甲狀腺素釋放激素(TRH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Plos one | Thyrotropin-Releasing Hormone (TRH) Promotes Wound Re-Epithelialisation in Frog and Human Skin [PubMed: PMC3759422] |
Analyst | Ultrasensitive detection of thyrotropin-releasing hormone based on azo coupling and surface-enhanced resonance Raman spectroscopy [Pubmed:27338554] |
journal of neuroendocrinology | Thyroid dysfunction in children with autism spectrum disorder is associated with folate receptor α autoimmune disorder. [pubmed:28199771] |
Journal of Neuroendocrinology | Thyroid dysfunction in children with autism spectrum disorder is associated with folate receptor α autoimmune disorder [10.1111/jne.12461] |
Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008] |
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