谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Transglutaminase 2 (TGM2)
tTG; TG2; C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase; Transglutaminase 2, Tissue; Transglutaminase C; Transglutaminase H
- 編號KSB830Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.625-40ng/mL
- 可適靈敏度0.227ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對組織轉(zhuǎn)谷氨酰胺酶(TGM2)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)活性蛋白 | Cell?culture;?Activity?Assays. |
PAB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體 | WB,IHC,ICC/IF |
LAB830Hu71 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAB830Hu22 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)單克隆抗體 | WB; IHC; ICC; IP. |
MEB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
CGB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)cDNA | PCR、蛋白表達等 |
參考文獻
雜志 | 參考文獻 |
Connective Tissue Research | Tissue transglutaminase is involved in mechanical load–induced osteogenic differentiation of human ligamentum flavum cells [Pubmed:27115725] |
American Journal of Respiratory Cell and Molecular Biology | ECM Crosslinking Enhances Fibroblast Growth and Protects Against Matrix Proteolysis in Lung Fibrosis. [pubmed:29053339] |
3 Biotech | Metformin attenuates TGF?β1?induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF?β pathways [Pubmed: 32550106] |
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