三葉因子1(TFF1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Trefoil Factor 1 (TFF1)
BCEI; HP1.A; HPS2; PS2; PNR2; Breast Cancer,Estrogen-Inducible Sequence Expressed In; Breast cancer estrogen-inducible protein; Polypeptide P1.A
- 編號KSB049Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.070ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對三葉因子1(TFF1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB049Mu01 | 三葉因子1(TFF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB049Mu01 | 三葉因子1(TFF1)多克隆抗體 | WB; IHC; ICC; IP. |
| SEB049Mu | 三葉因子1(TFF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB049Mu | 三葉因子1(TFF1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB049Mu01 | 三葉因子1(TFF1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| BMC Gastroenterology | Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in China [Biomedcentral: Source] |
| Biochimica et Biophysica Acta | miR218-5p regulates the proliferation of gastric cancer cells by targeting TFF1 in an Erk1/2-dependent manner [Pubmed:25652124] |
| clinical cancer research | Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma [PubMed: 26240291] |
| BMC?Cancer | miR-632 promotes gastric cancer progression by accelerating angiogenesis in a TFF1-dependent manner [Pubmed: 30612555] |
| Nutrients | Protective Effects of Wheat Peptides against Ethanol-Induced Gastric Mucosal Lesions in Rats: Vasodilation and Anti-Inflammation [Pubmed: 32784583] |
| International Journal of Molecular Sciences | Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid?… [] |
| PLOS MEDICINE | A combination of urinary biomarker panel and PancRISK score for earlier detection of pancreatic cancer: A case¨Ccontrol study [33301466] |
| Int. J. Mol. Sci. | Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨1ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und??- [] |
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