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心肌肌鈣蛋白T(TNNT2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Troponin T Type 2, Cardiac (TNNT2)

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T

  • 心肌肌鈣蛋白T(TNNT2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 心肌肌鈣蛋白T(TNNT2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對心肌肌鈣蛋白T(TNNT2)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

儲存

抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPD232Ra01 心肌肌鈣蛋白T(TNNT2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAD232Ra01 心肌肌鈣蛋白T(TNNT2)多克隆抗體 WB; IHC; ICC; IP.
MAD232Ra21 心肌肌鈣蛋白T(TNNT2)單克隆抗體 WB; IHC; ICC; IP.
MED232Ra 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
SED232Ra 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCD232Ra 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMD232Ra 心肌肌鈣蛋白T(TNNT2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSD232Ra01 心肌肌鈣蛋白T(TNNT2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志 參考文獻
Basic & Clinical Pharmacology & Toxicology Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in Rats [PubMed: 21232020]
Basic & Clinical Pharmacology & Toxicology Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats [Wiley: source]
European Journal of Pharmacology Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats [ScienceDirect: S0014299909009984]
Journal of translational medicine The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury [PubMed: PMC3694448]
Oral Diseases Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction [Pubmed: 22834943]
An International Journal of Pharmaceutical Sciences In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium [Pubmed: 23700796]
Journal of Animal and Feed Sciences, The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats [Pl:Source]
Liver Int. Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis [Pubmed:24330504]
Cardiovasc Toxicol. Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs [Pubmed:24420420]
British Journal of Pharmacology Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise [Pubmed:25039894]
Chemico-Biological Interactions Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways [Pubmed:26807765]
The Journal of Pharmacology and Experimental Therapeuties The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice [Pubmed:27247000]
The Journal of Maternal-Fetal & Neonatal Medicine Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies. [pubmed:27937003]
PLoS One. Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. [pubmed:27907155]
Biomedicine & Pharmacotherapy Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats [pubmed:28076830]
PLoS One Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model [pubmed:28570599]
EXPERIMENTAL AND THERAPEUTIC MEDICINE Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus [pubmed:29042932]
PLoS One Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury [Pubmed: 30192780]
PLoS One Tissue suction-mediated gene transfer to the beating heart in mice [Pubmed: 32027678]
Journal of Human Kinetics Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy [Pubmed: 32148580]
BIOMEDICINE & PHARMACOTHERAPY Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress [Pubmed: 33022535]
Toxics Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats [Pubmed: 32947820]
Elife Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice [33526170]
Cell Metabolism Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species [34536344]
Anal Bioanal Chem Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications [34673993]
Experimental and Therapeutic Medicine Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest [Pubmed:35495584]
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