類胰蛋白酶(TPS)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Tryptase (TPS)
TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
- 編號KSB070Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍15.6-1,000pg/mL
- 可適靈敏度5.5pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
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特異性
試劑盒中的抗體對類胰蛋白酶(TPS)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APB070Hu02 | 類胰蛋白酶(TPS)活性蛋白 | Cell?culture;?Activity?Assays. |
RPB070Hu01 | 類胰蛋白酶(TPS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB070Hu02 | 類胰蛋白酶(TPS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB070Hu01 | 類胰蛋白酶(TPS)多克隆抗體 | WB; IHC; ICC; IP. |
LAB070Hu71 | 類胰蛋白酶(TPS)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAB070Hu21 | 類胰蛋白酶(TPS)單克隆抗體 | WB; IHC; ICC; IP. |
MAB070Hu24 | 類胰蛋白酶(TPS)單克隆抗體 | WB,IHC |
MAB070Hu26 | 類胰蛋白酶(TPS)單克隆抗體 | WB,IHC |
MAB070Hu22 | 類胰蛋白酶(TPS)單克隆抗體 | WB; IHC |
MAB070Hu23 | 類胰蛋白酶(TPS)單克隆抗體 | WB; IHC. |
LAB070Hu76 | 類胰蛋白酶(TPS)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
FAB070Hu86 | 抗類胰蛋白酶(TPS)單克隆抗體(異硫氰酸熒光素標(biāo)記) | FCM |
WEB070Hu | 類胰蛋白酶(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEB070Hu | 類胰蛋白酶(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB070Hu | 類胰蛋白酶(TPS)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB070Hu | 類胰蛋白酶(TPS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSB070Hu01 | 類胰蛋白酶(TPS)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSB070Hu01 | 類胰蛋白酶(TPS)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Journal of Allergy and Clinical Immunology | Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps [PubMed: 22534535] |
J Pediatr. | Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 [Pubmed:24530123] |
Mediators of Inflammation | Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis [Pubmed:25400334] |
PLOS ONE | A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats [Pubmed:25541997] |
Neurogastroenterology & Motility | Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery [Pubmed:25677271] |
Mediators Inflamm | Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report [PubMed: 26089601] |
Int Immunopharmacol | Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis [PubMed: 26049029] |
Mediators of Inflammation | Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins [Journals: Mi] |
Mediators of Inflammation | Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins [Pubmed:27057098] |
Analytica Chimica Acta | An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis [] |
Journal of Clinical Investigation | Dengue virus-elicited tryptase induces endothelial permeability and shock [Pubmed: 31265436] |
Frontiers in Physiology | Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function [Pubmed: 31998139] |
Biomarkers of Allergic Asthma and their Association with Serum Parameters [] | |
Annals of Tropical Medicine & Public Health | Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity [] |
Front Immunol | Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive?… [34650557] |
Microbiol Spectr | Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome [Pubmed:35612327] |
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