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類胰蛋白酶(TPS)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Tryptase (TPS)

TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III

  • 類胰蛋白酶(TPS)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 類胰蛋白酶(TPS)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 類胰蛋白酶(TPS)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品圖片
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對類胰蛋白酶(TPS)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Allergy and Clinical Immunology Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps [PubMed: 22534535]
J Pediatr. Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 [Pubmed:24530123]
Mediators of Inflammation Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis [Pubmed:25400334]
PLOS ONE A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats [Pubmed:25541997]
Neurogastroenterology & Motility Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery [Pubmed:25677271]
Mediators Inflamm Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report [PubMed: 26089601]
Int Immunopharmacol Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis [PubMed: 26049029]
Mediators of Inflammation Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins [Journals: Mi]
Mediators of Inflammation Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins [Pubmed:27057098]
Analytica Chimica Acta An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis []
Journal of Clinical Investigation Dengue virus-elicited tryptase induces endothelial permeability and shock [Pubmed: 31265436]
Frontiers in Physiology Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function [Pubmed: 31998139]
Biomarkers of Allergic Asthma and their Association with Serum Parameters []
Annals of Tropical Medicine & Public Health Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity []
Front Immunol Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive?… [34650557]
Microbiol Spectr Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome [Pubmed:35612327]
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