酪氨酸羥化酶(TH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Tyrosine Hydroxylase (TH)
TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14
- 編號KSB438Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.066ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對酪氨酸羥化酶(TH)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPB438Hu01 | 酪氨酸羥化酶(TH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB438Hu01 | 酪氨酸羥化酶(TH)多克隆抗體 | WB; IHC; ICC; IP. |
MAB438Hu21 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
MAB438Hu24 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
MAB438Hu25 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
MAB438Hu22 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
SEB438Hu | 酪氨酸羥化酶(TH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB438Hu | 酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB438Hu01 | 酪氨酸羥化酶(TH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Mol Neurobiol. | 1, 25-dyhydroxyvitamin D3 Attenuates l-DOPA-Induced Neurotoxicity in Neural Stem Cells [Pubmed:25102940] |
PLOS ONE | Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome Sequencing [Pubmed:25181484] |
biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883] |
Therapeutics?and?Clinical?Risk?Management | Elevated?blood?plasma?levels?of?epinephrine,?norepinephrine,?tyrosine?hydroxylase, TGFβ1, and TNFα?associated?with?high-altitude?pulmonary?edema?in an Indian population. [pubmed:27540296] |
Sci Rep.? | Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. [pubmed:28230187] |
Journal of Cellular Biochemistry | Vaccarin administration ameliorates hypertension and cardiovascular remodeling in renovascular hypertensive rats [pubmed:28681939] |
Environmental?Science?& Technology | Multigenerational disruption of the thyroid endocrine system in marine medaka after a life-cycle exposure to perfluorobutanesulfonate [Pubmed:29565584] |
Molecular Neurobiology | Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD Model [Pubmed:29429051] |
Chemosphere | Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvae [Pubmed:29407805] |
Neurospine | The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem Cells [Pubmed:29656620] |
Toxicology and?Applied?Pharmacology | Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effects [Pubmed: 30222980] |
International Journal Of Cardiology | Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus [Pubmed: 30581101] |
Behavioural?Brain?Research | Hydroxychloroquine antiparkinsonian potential: Nurr1 modulation versus autophagy inhibition [] |
Environment International | Perfluoropolyether carboxylic acids (novel alternatives to PFOA) impair zebrafish posterior swim bladder development via thyroid hormone disruption [Pubmed: 31733528] |
Environmental Pollution | Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae [Pubmed: 31935612] |
International Journal of Pharmacology | Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease [Pubmed: 32084576] |
comparative biochemistry and physiology c-toxicology & pharmacology | Thyroid disruption and developmental toxicity caused by Cd2+ in Schizopygopsis younghusbandi larvae [Pubmed: 32344129] |
International Journal of Stem cell Research & Therapy | Selection of Cells for Parkinson's Disease Cell-Therapy [] |
Ecotoxicol Environ Saf | Effects of SiO2 nanoparticles on the uptake of tetrabromobisphenol A and its impact on the thyroid endocrine system in zebrafish larvae [33385677] |
Nutr Res | Tocotrienols protect differentiated SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine-induced cytotoxicity by ameliorating dopamine biosynthesis and?… [Pubmed:35065349] |
Food Chem Toxicol | Omarigliptin attenuates rotenone-induced Parkinson's disease in rats: Possible role of oxidative stress, endoplasmic reticulum stress and immune modulation [Pubmed:35439590] |
Food Chemistry: Molecular Sciences | Unravelling the neuroprotective mechanisms of carotenes in differentiated human neural cells: biochemical and proteomic approaches [Pubmed:35415676] |
Journal of Environmental Sciences | Parental and transgenerational impairments of thyroid endocrine system in zebrafish by 2, 4, 6-tribromophenol [] |
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