Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)
P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide
- 編號SEA957Hu
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍78-5,000pg/mL
- 靈敏度最小可檢測劑量小于等于30pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2940 ¥ 4200 ¥ 18900 ¥ 35700 ¥ 294000
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特異性
本試劑盒用于檢測Ⅰ型前膠原氨基端原肽(PINP),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅰ型前膠原氨基端原肽(PINP)(加標樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 83-99 | 91 |
EDTA plasma(n=5) | 93-103 | 97 |
heparin plasma(n=5) | 88-95 | 92 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Ⅰ型前膠原氨基端原肽(PINP),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中Ⅰ型前膠原氨基端原肽(PINP)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-92% | 84-91% | 82-99% | 80-91% |
EDTA plasma(n=5) | 85-101% | 94-101% | 81-98% | 83-92% |
heparin plasma(n=5) | 91-99% | 80-91% | 87-96% | 88-101% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。
實驗原理
將Ⅰ型前膠原氨基端原肽(PINP)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的Ⅰ型前膠原氨基端原肽(PINP)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的Ⅰ型前膠原氨基端原肽(PINP)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的Ⅰ型前膠原氨基端原肽(PINP)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA957Hu02 | Ⅰ型前膠原氨基端原肽(PINP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA957Hu61 | Ⅰ型前膠原氨基端原肽(PINP)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA957Hu61 | Ⅰ型前膠原氨基端原肽(PINP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
SPA957Hu02 | Ⅰ型前膠原氨基端原肽(PINP)合成多肽 | Immunogen; Blocking Peptide. |
CPA957Hu12 | Ⅰ型前膠原氨基端原肽(PINP)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu32 | Ⅰ型前膠原氨基端原肽(PINP)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu31 | Ⅰ型前膠原氨基端原肽(PINP)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu22 | Ⅰ型前膠原氨基端原肽(PINP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu23 | Ⅰ型前膠原氨基端原肽(PINP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA957Hu21 | Ⅰ型前膠原氨基端原肽(PINP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
SPA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)合成多肽 | Immunogen; Blocking Peptide. |
CPA957Hu11 | Ⅰ型前膠原氨基端原肽(PINP)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA957Hu08 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | WB; IHC; ICC; IP. |
PAA957Hu09 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | WB; IHC; ICC; IP. |
PAA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | WB; IHC; ICC; IP. |
PAA957Hu02 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | WB; IHC; ICC; IP. |
LAA957Hu81 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
LAA957Hu71 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAA957Hu22 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB |
RAA957Hu23 | Ⅰ型前膠原氨基端原肽(PINP)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
RAA957Hu22 | Ⅰ型前膠原氨基端原肽(PINP)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
MAA957Hu24 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA957Hu21 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB,IHC |
MAA957Hu28 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB; IHC |
MAA957Hu23 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA957Hu25 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA957Hu27 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA957Hu29 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB; IHC; ICC; IP. |
SEA957Hu | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
IEA957Hu | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA957Hu | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA957Hu | Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA957Hu11 | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
KSA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
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