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Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Procollagen I N-Terminal Propeptide (PINP) ,etc. by FLIA (Flow Luminescence Immunoassay)

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

（注：單次混測多因子不超過8個指標 ）

  • Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-90 80
EDTA plasma(n=5) 93-105 96
heparin plasma(n=5) 87-95 91
sodium citrate plasma(n=5) 93-101 98

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 92-104% 81-94% 82-95%
EDTA plasma(n=5) 78-94% 85-103% 85-99% 80-97%
heparin plasma(n=5) 98-105% 86-102% 89-98% 83-104%
sodium citrate plasma(n=5) 78-93% 93-102% 84-97% 78-90%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
    37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數。

實驗原理

將Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結合，然后將未結合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。

贈品

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編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
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