二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Amiloride Binding Protein 1 (ABP1) ,etc. by FLIA (Flow Luminescence Immunoassay)
AOC1; DAO; KAO; Histaminase; Kidney amine oxidase; Diamine Oxidase; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號LMA656Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.04-40ng/mL
- 靈敏度最小可檢測劑量小于等于0.013 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-103 | 99 |
| EDTA plasma(n=5) | 80-102 | 87 |
| heparin plasma(n=5) | 98-105 | 102 |
| sodium citrate plasma(n=5) | 78-97 | 82 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-94% | 86-101% | 93-101% | 83-101% |
| EDTA plasma(n=5) | 93-101% | 91-99% | 94-101% | 98-105% |
| heparin plasma(n=5) | 87-101% | 88-97% | 97-104% | 79-96% |
| sodium citrate plasma(n=5) | 90-101% | 94-102% | 97-105% | 78-94% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
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| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA656Ra02 | 二胺氧化酶(ABP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA656Ra01 | 二胺氧化酶(ABP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA656Ra02 | 二胺氧化酶(ABP1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA656Ra01 | 二胺氧化酶(ABP1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA656Ra71 | 二胺氧化酶(ABP1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA656Ra22 | 二胺氧化酶(ABP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA656Ra21 | 二胺氧化酶(ABP1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA656Ra | 二胺氧化酶(ABP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA656Ra | 二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Applied Clay Science | Effects of copper-exchanged montmorillonite, as alternative to antibiotic, on diarrhea, intestinal permeability and proinflammatory cytokine of weanling pigs [Cas.zju: Source] |
| Phytomedicine.? | A multi-component herbal preparation, STW 5, shows anti-apoptotic effects in radiation induced intestinal mucositis in rats [Pubmed:25022208] |
| Oxid Med Cell Longev | Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation Model [PubMed: 26064429] |
| Pancreatology. | Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. [Pubmed:26804005] |
| ?Biochimica et Biophysica Acta (BBA)-General Subjects | Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485] |
| Journal of Controlled Release | A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195] |
| Advances in Experimental Medicine and Biology | Serum Diamine Oxidase in Pseudoallergy in the Pediatric Population [pubmed:28804811] |
| PLoS One | Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats [pubmed:28622357] |
| Nutrients | Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron [Pubmed:29861488] |
| Scientific Reports | Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsia [Pubmed:29679053] |
| British Journal of Nutrition | Postoperative symbiotic in patients with head and neck cancer: a double-blind randomised trial [Pubmed:29277158] |
| Food?&?Function | The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer [Pubmed:29384543] |
| 碩士論文 | LOCALIZATION OF COPPER-CONTAINING AMINE OXIDASE ENZYMES IN THE BLOOD [] |
| International?Journal?of?Clinical?and?Experimental?Medicine | Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease [] |
| Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease [] |
| Acta Diabetologica | Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated mice [Pubmed: 31749050] |
| Biomed Research International | Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099] |
| FASEB JOURNAL | HMGB1‐associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in rats [Pubmed: 31961020] |
| JOURNAL OF ETHNOPHARMACOLOGY | The effect of Poria cocos ethanol extract on the intestinal barrier function and intestinal microbiota in mice with breast cancer [Pubmed: 33039631] |
| Cell Death Discovery | Fecal microbiota transplantation from patients with autoimmune encephalitis modulates Th17 response and relevant behaviors in mice [Pubmed: 32821439] |
| The Journal of Nutritional Biochemistry | Effects of fucoidan on tumor prevention and gut flora [] |
| Chemical Biology & Dug Design | Design and development of novel fasudil derivatives as potent anti‐breast cancer agent that improves intestinal flora and intestinal barrier function in rats [34587363] |
| Journal of Functional Foods | Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways [] |
| Stem Cell Research & Therapy | Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model [] |
