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血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Angiopoietin 2 (ANGPT2) ,etc. by FLIA (Flow Luminescence Immunoassay)

ANG2; AGPT2

（注：單次混測多因子不超過8個指標 ）

  • 血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • 血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-102 83
EDTA plasma(n=5) 82-102 88
heparin plasma(n=5) 83-95 86

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 95-102% 84-96% 94-105%
EDTA plasma(n=5) 87-101% 87-101% 89-101% 86-94%
heparin plasma(n=5) 82-104% 89-104% 88-102% 80-91%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應用(僅供研究使用，不用于臨床診斷！)
RPA009Ra01 血管生成素2(ANGPT2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA009Ra02 血管生成素2(ANGPT2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA009Ra02 血管生成素2(ANGPT2)多克隆抗體 WB; IHC; ICC; IP.
PAA009Ra01 血管生成素2(ANGPT2)多克隆抗體 WB; IHC; ICC; IP.
LAA009Ra71 血管生成素2(ANGPT2)多克隆抗體(生物素標記) WB; IHC; ICC.
SEA009Ra 血管生成素2(ANGPT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA009Ra 血管生成素2(ANGPT2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Microvascular Research Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle [PubMed: 20937289]
Theriogenology Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article]
Pediatr Infect Dis J Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean–Congo Hemorrhagic Fever [PubMed: 25831422]
Oncotarget Long noncoding RNA hotair mediated angiogenesis in nasopharyngeal carcinoma by direct and indirect signaling pathways [Pubmed:26717040]
Experimental?Animals Ginsenoside?Rb1?improves?cardiac?function?and?remodeling?in?heart?failure. [pubmed:28367863]
Sci Rep.? Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491]
PeerJ? The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker [pubmed:28584715]
Cellular Physiology and Biochemistry Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats [pubmed:28848201]
Tumor Biology Cervical cancer cell–derived angiopoietins promote tumor progression [pubmed:28720059]
Anesthesiology Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in?… [Pubmed:28968277]
British Journal of Anaesthesia Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats [10.1016:j.bja.2018.05.049]
Anticancer?Research Oligodendrocytes Up-regulate the Invasive Activity of Glioblastoma Cells via the Angiopoietin-2 Signaling Pathway [Pubmed: 30711932]
Comparative Medicine Characterization of a Porcine Model for Von Willebrand Disease Type 1 and 3 Regarding Expression of Angiogenic Mediators in the Nonpregnant Female?… [Pubmed: 31526432]
BMC Endocrine Disorders The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children [Pubmed: 31771561]
Biology of Reproduction Expression of angiogenic factors in the uteroplacental unit is altered at time of placentation in a porcine model of von Willebrand disease type 1 [Pubmed: 31806575]
Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827]
MEDIATORS OF INFLAMMATION Mica Can Alleviate TNBS-Induced Colitis in Mice by Reducing Angiotensin II and IL-17A and Increasing Angiotensin-Converting Enzyme 2, Angiotensin 1-7?… [Pubmed: 33100902]
Scientific Reports Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735]
Intensive Care Med Exp The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats [33997943]
Gynecol Endocrinol Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study [34282706]
Clinical and Experimental Immunology Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients [Pubmed:35348620]
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