促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Corticotropin Releasing Factor (CRF) ,etc. by FLIA (Flow Luminescence Immunoassay)
CRH; Corticotropin Releasing Hormone; Corticoliberin
（注：單次混測多因子不超過8個指標 ）
- 編號LMA835Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長1.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型Serum, plasma and other biological fluids
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- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 84-92 | 88 |
| EDTA plasma(n=5) | 92-99 | 96 |
| heparin plasma(n=5) | 91-98 | 95 |
| sodium citrate plasma(n=5) | 95-102 | 99 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-95% | 82-105% | 94-101% | 95-105% |
| EDTA plasma(n=5) | 97-105% | 93-105% | 84-101% | 78-104% |
| heparin plasma(n=5) | 95-102% | 94-101% | 85-105% | 92-101% |
| sodium citrate plasma(n=5) | 98-105% | 84-98% | 97-104% | 79-90% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結合，然后將未結合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。
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相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA835Mu01 | 促腎上皮質激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA835Mu02 | 促腎上皮質激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA835Mu21 | 促腎上皮質激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA835Mu22 | 促腎上皮質激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA835Mu33 | 促腎上皮質激素釋放激素(CRF)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA835Mu09 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA835Mu01 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA835Mu02 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA835Mu07 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA835Mu08 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA835Mu81 | 促腎上皮質激素釋放激素(CRF)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA835Mu71 | 促腎上皮質激素釋放激素(CRF)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MEA835Mu | 促腎上皮質激素釋放激素(CRF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA835Mu | 促腎上皮質激素釋放激素(CRF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA835Mu | 促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA835Mu11 | 促腎上皮質激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345] |
| Bipolar?Disord. | Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β? [Pubmed:24862833] |
| Peptides | Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level [Pubmed:25236411] |
| Ann Dermatol | Acute?Stress-Induced?Changes?in?Follicular?Dermal?Papilla?Cells?and?Mobilization?of?Mast?Cells:Implications?for?Hair?Growth. [pubmed:27746640] |
| Neuroscience | Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation [pubmed:28663093] |
| Biomed Research International | Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation [pubmed:29082242] |
| Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats [Pubmed:30028991] |
| Frontiers in Pharmacology | Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary?… [Pubmed:30038568] |
| Toxicology Letters | Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors [Pubmed:29807116] |
| Neuropsychiatric disease and treatment | The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic?… [Pubmed:29379286] |
| BMC?Veterinary?Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine?… [Pubmed:29540178] |
| Bioscience Research | Bioscience Research [] |
| FASEB Journal | Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice [Pubmed: 31431066] |
| Aging Cell | Altered glucocorticoid metabolism represents a feature of macroph‐aging [Pubmed: 32463582] |
| Endocr J | Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress [33408312] |
| CRYOBIOLOGY | O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress [33651993] |
