脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Fatty Acid Binding Protein 1 (FABP1) ,etc. by FLIA (Flow Luminescence Immunoassay)
FABP-1; FABPL; L-FABP; LFABP; Liver-type fatty acid-binding protein; Fatty Acid Binding Protein 1, Liver
(注:單次混測多因子不超過8個指標 )
- 編號LMB566Ga
- 物種Chicken (Gallus,雞) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.05-50ng/mL
- 靈敏度最小可檢測劑量小于等于0.017 ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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特異性
本試劑盒用于檢測脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 86-95 | 89 |
EDTA plasma(n=5) | 79-97 | 90 |
heparin plasma(n=5) | 81-96 | 88 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 98-105% | 91-102% | 91-101% | 89-96% |
EDTA plasma(n=5) | 93-105% | 85-103% | 80-99% | 92-105% |
heparin plasma(n=5) | 99-105% | 86-96% | 91-105% | 93-101% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠)標準品或樣本100μL及磁珠10μL,
37°C酶標板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠,其中的脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體,將未結(jié)合的生物素化抗體洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Chicken (Gallus,雞) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB566Ga01 | 脂肪酸結(jié)合蛋白1(FABP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB566Ga01 | 脂肪酸結(jié)合蛋白1(FABP1)多克隆抗體 | WB; IHC; ICC; IP. |
LAB566Ga71 | 脂肪酸結(jié)合蛋白1(FABP1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
LAB566Ga81 | 脂肪酸結(jié)合蛋白1(FABP1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
SEB566Ga | 脂肪酸結(jié)合蛋白1(FABP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB566Ga | 脂肪酸結(jié)合蛋白1(FABP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB566Ga01 | 脂肪酸結(jié)合蛋白1(FABP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
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Cryobiology.? | Ex vivo use of a Rho-kinase inhibitor during renal preservation improves graft function upon reperfusion [Pubmed:25555715] |
Clin Transl Sci | Controlled Rewarming after Hypothermia: Adding a New Principle to Renal Preservation [PubMed: 26053383] |
SAGE journals | The role of serum I-FABP concentration in assessment of small intestine mucosa among HIV-infected patients [Content: Early] |
Transplant International | Kidney transplantation after oxygenated machine perfusion preservation with Custodiol‐N solution [PubMed: 25882869] |
European?Journal of?Clinical?Investigation | Prediction of renal function upon reperfusion by ex situ controlled oxygenated rewarming [pubmed:27718228] |
Journal of?Clinical?and Diagnostic?Research? | The Importance of Liver-Fatty Acid Binding Protein in Diagnosis of Liver Damage in Patients with Acute Hepatitis [pubmed:28571184] |
Annals of Nutrition and Metabolism | Plasma Free Fatty Acids and their Binding Proteins in Preterm Infants [Pubmed:30045009] |
Scandinavian Journal of?Clinical?and Laboratory Investigation | Isolated kidney perfusion: the influence of pulsatile flow [Pubmed:29301417] |
International Journal of Medical Sciences | Circulating fatty acid-binding protein 1 (FABP1) and nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus [Pubmed: 32038102] |
International Journal of Medical Sciences | FABP1 and FABP2 as markers of diabetic nephropathy [Pubmed: 32922200] |
Archives of Clinical and Biomedical Research | Profile of Intestinal Barrier Functional Markers in Italian Patients with Diarrhea-Predominant IBS: Preliminary Data from a Low-FODMAPs diet Trial [] |
Antioxidants | The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males [34071378] |
antioxidants | TCA Cycle and Fatty Acids Oxidation Reflect Early Cardiorenal Damage in Normoalbuminuric Subjects with Controlled Hypertension [34356333] |
Association of plasma fatty acid-binding protein 3 with estimated glomerular filtration rate in patients with type 2 diabetes mellitus [34975301] |