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腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2) ,etc. by FLIA (Flow Luminescence Immunoassay)

FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein

（注：單次混測多因子不超過8個指標 ）

  • 腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 80-99 93
heparin plasma(n=5) 86-94 89
sodium citrate plasma(n=5) 82-92 85

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 94-105% 78-96% 96-104% 82-98%
EDTA plasma(n=5) 95-103% 87-102% 80-88% 93-101%
heparin plasma(n=5) 84-98% 78-103% 95-103% 89-102%
sodium citrate plasma(n=5) 93-101% 89-103% 92-105% 90-104%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Sus scrofa; Porcine (Pig，豬) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA559Po01 腸型脂肪酸結(jié)合蛋白(FABP2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA559Po01 腸型脂肪酸結(jié)合蛋白(FABP2)多克隆抗體 WB; IHC; ICC; IP.
SEA559Po 腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA559Po 腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Food Chem Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes [PubMed: 25952883]
Oxid Med Cell Longev Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation Model [PubMed: 26064429]
Vet Immunol Immunopathol Immunomodulatory potential of β-glucan as supportive treatment in porcine rotavirus enteritis. [pubmed:28895864]
PLoS One Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats [pubmed:28622357]
Journal of Immunology and Immunopathology Effect of Porcine Rotavirus Enteritis on Concentrations of Interferonγ, Immunoglobulin-G and Intestinal Fatty Acid-Binding Protein-2 and Peripheral Leukocyte Function [10.5958/0973-9149.2017.00011.9]
Cellular Physiology and Biochemistry Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory?… [Pubmed:29672286]
European?Journal of Clinical Nutrition Lack of liver steatosis in germ-free mice following hypercaloric diets [Pubmed:29926176]
Nutrients Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron [Pubmed:29861488]
International?Surgery?Journal Intestinal fatty acid binding protein (I-FABP) as a marker for acute intestinal ischemia [Doi: 10.18203/2349-2902.isj20190058]
Hepatology?Communications Effect of a Multistrain Probiotic on Cognitive Function and Risk of Falls in Patients With Cirrhosis: A Randomized Trial []
Tropical?Animal?Health?and?Production Immunomodulating dose of levamisole stimulates innate immune response and prevents intestinal damage in porcine rotavirus diarrhea: a restricted-randomized?… [Pubmed: 30790158]
Biological & Pharmaceutical Bulletin Role of Aquaporin-3 in Intestinal Injury Induced by Sepsis [Pubmed: 31582652]
International Journal of Medical Sciences FABP1 and FABP2 as markers of diabetic nephropathy [Pubmed: 32922200]
Int Immunopharmacol Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response [Pubmed: 32361189]
Journal of Exercise Physiology Online Markers of Intestinal Damage in Individuals with and without Obesity during a 12-Week Exercise Period. []
The Journal of Nutritional Biochemistry Effects of fucoidan on tumor prevention and gut flora []
european journal of clinical nutrition? Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury [33514869]
J Cell Mol Med The |á2AR/Caveolin?\1/p38MAPK/NF?\|êB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia?\reperfusion [34114328]
BMC Gastroenterol Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity [34022802]
JOURNAL OF APPLIED PHYSIOLOGY Impact of Successive Exertional Heat Injuries on Thermoregulatory and Systemic Inflammatory Responses in Mice [34528459]
PLoS One The protective effects of HIF-1α activation on sepsis induced intestinal mucosal barrier injury in rats model of sepsis [Pubmed:35576220]
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