国产成人三级网址在线看_日韩高清在线毛片_精品国内自产拍在线观看尤物_欧美精品 亚洲日韩_成人黄色影片在线看_国产一区二区播放_国产午夜理论最新电影_日本伊人久久综合_亚洲国产网站视频在线播放_日韩人妻系列无码专区

G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for G Protein Coupled Estrogen Receptor 1 (GPER) ,etc. by FLIA (Flow Luminescence Immunoassay)

GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1

（注：單次混測多因子不超過8個指標 ）

  • G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-104 88
EDTA plasma(n=5) 79-94 88
heparin plasma(n=5) 78-99 83
sodium citrate plasma(n=5) 92-105 101

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 88-95% 88-96% 90-102%
EDTA plasma(n=5) 85-99% 78-104% 92-101% 89-103%
heparin plasma(n=5) 88-98% 86-93% 98-105% 85-96%
sodium citrate plasma(n=5) 85-94% 86-97% 85-102% 83-95%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。

實驗原理

將G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產品

編號 適用物種：Mus musculus (Mouse，小鼠) 應用(僅供研究使用，不用于臨床診斷！)
RPG045Mu01 G蛋白偶聯雌激素受體1(GPER)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPG045Mu02 G蛋白偶聯雌激素受體1(GPER)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAG045Mu01 G蛋白偶聯雌激素受體1(GPER)多克隆抗體 WB; IHC; ICC; IP.
PAG045Mu02 G蛋白偶聯雌激素受體1(GPER)多克隆抗體 WB; IHC; ICC; IP.
SEG045Mu G蛋白偶聯雌激素受體1(GPER)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG045Mu G蛋白偶聯雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
The Journal of Steroid Biochemistry and Molecular Biology Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling [Pubmed:24846829]
Molecular and Cellular Endocrinology Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells [Pubmed:24859647]
J Steroid Biochem Mol Biol Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR32 signalling [PubMed: 24846831]
Journal of Environmental Sciences Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts [science:S1001074216300924]
Psychiatry research Serum levels of G protein-coupled estrogen receptor 1 (GPER1) in drug-naive patients with generalized anxiety disorder. [pubmed:27512921]
Clinical Psychopharmacology Neuroscience. Increased Serum G Protein-coupled Estrogen Receptor 1 Levels and Its Diagnostic Value in Drug Na?ve Patients with Major Depressive Disorder [pubmed:29073745]
Psychiatry?Research Decreased levels of G protein-coupled estrogen receptor in children with autism spectrum disorders. [pubmed:28734238]
Neuropsychiatric disease and treatment serum levels of gPer-1 in euthymic bipolar patients [Pubmed:29618927]
European archives of oto-rhino-laryngology An evaluation of G-protein coupled membrane estrogen receptor-1 level in stuttering [Pubmed:29299745]
Bosnian Journal of?Basic?Medical?Sciences Evaluation of estrogen and G protein-coupled estrogen receptor 1 (GPER) levels in drug-na?ve patients with attention deficit hyperactivity disorder (ADHD) [Pubmed:29659348]
Carleton University Research Virtual Environment In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts []
Am J Otolaryngol GPER-1 and sex-hormone levels in patients with otosclerosis [Pubmed: 32144019]
Andrologia. Relationship between the G protein–coupled oestrogen receptor and spermatogenesis, and its correlation with male infertility [Pubmed: 32776559]
Journal of Radiation Research and Applied Sciences GPER-1 in chronic hepatitis B []
FASEB J Endocrine disruption in Crohn's disease: Bisphenol A enhances systemic inflammatory response in patients with gut barrier translocation of dysbiotic microbiota??- [34085740]
Comparing the Impact of Serum GPER-1 and Oxidant/Antioxidant Levels on Retinopathy in Diabetic Patients and Healthy Individuals: A Pilot Study []
Horm Mol Biol Clin Investig The change pattern in serum G protein-coupled estrogen receptor-1 (GPER1) levels during pregnancy with and without gestational diabetes mellitus [34787384]
留言咨詢