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干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Interferon Gamma (IFNg) ,etc. by FLIA (Flow Luminescence Immunoassay)

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

(注:?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) )

  • 干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法),經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 78-90 85
EDTA plasma(n=5) 93-102 97
heparin plasma(n=5) 87-101 98
sodium citrate plasma(n=5) 84-98 91

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線性范圍即為稀釋后樣本中干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 80-96% 87-95% 84-92% 87-103%
EDTA plasma(n=5) 85-94% 83-96% 94-101% 80-90%
heparin plasma(n=5) 97-105% 78-91% 86-96% 98-105%
sodium citrate plasma(n=5) 94-105% 82-99% 95-104% 93-101%

穩(wěn)定性

經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品、樣本、磁珠)標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL,
    37°C酶標(biāo)板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測(cè)溶液A100μL,37°C酶標(biāo)板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測(cè)溶液B100μL,37°C振動(dòng)孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠,其中的干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體,將未結(jié)合的生物素化抗體洗凈后,加入PE標(biāo)記的親和素,再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈(zèng)品

相關(guān)產(chǎn)品

編號(hào) 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
APA049Ra01 干擾素γ(IFNg)活性蛋白 Cell?culture;?Activity?Assays.
EPA049Ra61 干擾素γ(IFNg)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA049Ra02 干擾素γ(IFNg)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA049Ra61 干擾素γ(IFNg)活性蛋白 Cell?culture;?Activity?Assays.
RPA049Ra01 干擾素γ(IFNg)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA049Ra01 干擾素γ(IFNg)多克隆抗體 WB; IHC; ICC; IP.
PAA049Ra02 干擾素γ(IFNg)多克隆抗體 WB; IHC; ICC; IP.
PAA049Ra06 干擾素γ(IFNg)多克隆抗體 WB; IHC; ICC; IP.
LAA049Ra81 干擾素γ(IFNg)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAA049Ra71 干擾素γ(IFNg)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA049Ra22 干擾素γ(IFNg)單克隆抗體 WB; IHC; ICC; IP.
MAA049Ra23 干擾素γ(IFNg)單克隆抗體 WB; IHC; ICC; IP.
MAA049Ra21 干擾素γ(IFNg)單克隆抗體 WB; IHC; ICC; IP.
MEA049Ra 干擾素γ(IFNg)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA049Ra 干擾素γ(IFNg)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA049Ra 干擾素γ(IFNg)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA049Ra 干擾素γ(IFNg)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA049Ra01 干擾素γ(IFNg)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".
CGA049Ra01 干擾素γ(IFNg)cDNA PCR、蛋白表達(dá)等

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Free Radical Biology and Medicine Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat [Pubmed: 31255668]
Animals Anti-Inflammatory State in Arabian Horses Introduced to the Endurance Training [Pubmed: 31462005]
Infection and Drug Resistance Disseminated And Infection Accompanied Sweet's Syndrome In A Patient With Anti-Interferon-γ Autoantibodies: A Case Report. [Pubmed: 31632104]
Open Forum Infectious Diseases Characterization of Anti–Interferon-γ Antibodies in HIV-Negative Patients Infected With Disseminated and Cryptococcosis. [Pubmed: 31660325]
Polish Journal of Veterinary Sciences Effect of postpartum administration of ketoprofen on proinflammatory cytokine concentration and their correlation with lipogenesis and ketogenesis in Holstein?… [Pubmed: 31560476]
Experimental Cell Research CD4+ CD25+ Tregs as dependent factor in the course of bleomycin-induced pulmonary fibrosis in mice [Pubmed: 31678213]
International Immunopharmacology Melatonin alleviates oxidative stress in sleep deprived mice: Involvement of small intestinal mucosa injury [Pubmed: 31835081]
International Journal of Rheumatic Diseases Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosis [Pubmed: 31840939]
Frontiers in Immunology The Increase in IL-1β in the Early Stage of Heatstroke Might Be Caused by Splenic Lymphocyte Pyroptosis Induced by mtROS-Mediated Activation of the?… [Pubmed: 31921131]
Nutrients Rotundic Acid Protects against Metabolic Disturbance and Improves Gut Microbiota in Type 2 Diabetes Rats [Pubmed: 31887996]
Transboundary and Emerging Diseases Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China [Pubmed: 32187856]
Nanomedicine The toxicity of alumina nanoparticles to immune system in mice [Pubmed: 32162999]
Scientific Reports Serum sHLA-G: Significant diagnostic biomarker with respect to therapy and immunosuppressive mediators in Head and Neck Squamous Cell Carcinoma [Pubmed: 32123232]
American Journal of Translational Research Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193]
Journal of Inflammation Research Active Cousinia thomsonii Extracts Modulate Expression of Crucial Proinflammatory Mediators/Cytokines and NFκB Cascade in Lipopolysaccharide-Induced?… [Pubmed: 33173324]
Toxicol Appl Pharmacol Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling [Pubmed: 33171191]
MOLECULAR AND CELLULAR BIOCHEMISTRY Ectopic expression of 35 kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMT [Pubmed: 33083950]
BIOMEDICINE & PHARMACOTHERAPY Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress [Pubmed: 33022535]
DRUG AND CHEMICAL TOXICOLOGY Toxicological impact of sodium benzoate on inflammatory cytokines, oxidative stress and biochemical markers in male Wistar rats [Pubmed: 33003960]
Life Sci PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape [Pubmed: 32971102]
Vet Res Construction of polycistronic baculovirus surface display vectors to express the PCV2 Cap (d41) protein and analysis of its immunogenicity in mice and swine [Pubmed: 32907618]
Int Immunopharmacol BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice [Pubmed: 32717566]
EXPERIMENTAL DERMATOLOGY Decreased suppression of CD8+ and CD4+ T cells by peripheral Regulatory T cells in Generalized Vitiligo due to reduced NFATC1 and FOXP3 proteins [Pubmed: 32682346]
Parasites & Vectors Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism?… [Pubmed: 32680553]
Life Sci Impact of diminazene aceturate on renin-angiotensin system, infectious myocarditis and skeletal myositis in mice: An in vitro and in vivo study [Pubmed: 32652140]
cancer immunology immunotherapy TIM-3 blockade combined with bispecific antibody MT110 enhances the anti-tumor effect of γδ T cells [Pubmed: 32588076]
BIOLOGICAL TRACE ELEMENT RESEARCH Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225]
Molecular Medicine Reports Aquaporin?4 deletion ameliorates enterovirus 71 infection in mice [Pubmed: 32582978]
BIOTECHNOLOGY LETTERS Recombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF-κB pathway [Pubmed: 32567013]
Int Immunopharmacol Celastrol attenuates collagen-induced arthritis via inhibiting oxidative stress in rats [Pubmed: 32402948]
Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra []
Journal of Functional Foods Polyphenolic extracts from Wushan tea leaves attenuate hepatic injury in CCl4-treated mice []
Indian Journal of Animal Research Immunological Effects of a Single Dose of PLGA Nanoparticles Encapsulated Peptide in Broilers in Comparison to Traditional Vaccines against Infectious Bursal … []
Hormonal and Cytokine Blood Profile of Dairy Cows in the Early Gestation Period []
Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction by enhancing Th2 cytokine []
Scientific Reports Immunogenicity and protective efficacy of enterotoxigenic Escherichia coli (ETEC) total RNA against ETEC challenge in a mouse model [33239756]
Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512]
Eur Rev Med Pharmacol Sci Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients [33755963]
Biomed Pharmacother Macro- and microtranscriptomic evidence of the monocyte recruitment to regenerating liver after partial hepatectomy in mouse model [33765583]
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928]
Life Sciences p-Coumaric acid ameliorates ionizing radiation-induced intestinal injury through modulation of oxidative stress and pyroptosis [33915129]
Infect Drug Resist Talaromyces marneffei and Burkholderia cepacia Co-Infection in a HIV-Uninfected Patient with Anti-Interferon-|? Autoantibodies [34140785]
Vaccines Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious??- [33670249]
Pharmaceutics PK/PD Modeling of the PDE7 Inhibitor?aGRMS-55 in a Mouse Model of Autoimmune Hepatitis. Pharmaceutics 2021, 13, 597 [33919375]
Melatonin-mediated MT2 attenuates colitis induced by dextran sodium sulfate via PI3K/AKT/Nrf2/SIRT1/ROR|á/NF-|êB signaling pathways [34162146]
How Does the Th1/Th2 Polarization in Milk Change According to the Types of Bacteria that Cause Subclinical Mastitis in Cows? []
Amplified Antitumor Efficacy By A Targeted Drugs Retention and Chemosensitization Strategies-Based ?°Combo?± Nanoagent Together With PD-L1 Blockade In??- []
World J Tradit Chin Med Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancement []
Cancer Lett Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis [34256094]
J Pharm Pharmacol Alamandine protects against renal ischaemia–reperfusion injury in rats via inhibiting oxidative stress [34244746]
J Nanobiotechnology Amplified antitumor efficacy by a targeted drug retention and chemosensitization strategy-based “combo” nanoagent together with PD-L1 blockade in?… [34225744]
Neoplasia PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1 [34325342]
Sci Rep Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients [34376749]
Am J Transl Res Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237]
J Immunol Res Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633]
Sci Rep Novel interferon-sensitive genes unveiled by correlation-driven gene selection and systems biology [34508139]
Transl Psychiatry Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive?… [34802039]
Infect Drug Resist Retrospective Analysis of 10 Cases of Disseminated Nontuberculous Mycobacterial Disease with Osteolytic Lesions [34785914]
Environmental Research In-vitro oxidative potential and inflammatory response of ambient PM2. 5 in a rural region of Northwest China: Association with chemical compositions and?… [34863982]
Eur J Pharm Sci Preclinical study of methotrexate-based hydrogels versus surfactant based liquid crystal systems on psoriasis treatment [34314841]
J Inflamm Res Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice [Pubmed:35058704]
Poult Sci Salmonella phage CKT1 significantly relieves the body weight loss of chicks by normalizing the abnormal intestinal microbiome caused by hypervirulent?… [Pubmed:35063807]
ACS Appl Mater Interfaces Combinational Chemoimmunotherapy for Breast Cancer by Codelivery of Doxorubicin and PD-L1 siRNA Using a PAMAM-Incorporated Liposomal Nanoplatform [Pubmed:35138103]
Theranostics Combating multidrug resistance and metastasis of breast cancer by endoplasmic reticulum stress and cell-nucleus penetration enhanced?… [Pubmed:35401832]
Inflammopharmacology Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway [Pubmed:35366745]
Journal of Nanobiotechnology Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM [Pubmed:35351131]
Infection and Drug Resistance Sweet's Syndrome Accompanied by Coinfection with Multiple Pathogens and Disseminated Mycobacterium phlei Infection Presenting with Osteolytic?… [Pubmed:35592103]
Sci Rep Clinical findings and predictive factors for positive anti-interferon-γ autoantibodies in patients suffering from a non-tuberculosis mycobacteria or Talaromyces?… [Pubmed:35641599]
Biomedicines Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model [Pubmed:35740356]
Viruses Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs [Pubmed:35215787]
Antioxidants Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress [Pubmed:35204158]
Oxidative Medicine and Cellular Longevity The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced Colitis [Pubmed:35355860]
Journal of Integrative Medicine Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats [Pubmed:35643766]
researchsquare MSC-EVs: A Promising Modulator for Immune Response in Systemic Lupus Erythematosus []
Mljekarstvo Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows []
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