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粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Mucin 5 Subtype AC (MUC5AC) ,etc. by FLIA (Flow Luminescence Immunoassay)

TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein

（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）

  • 粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈(zèng)品

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編號(hào) 適用物種：Rattus norvegicus (Rat，大鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA756Ra01 粘蛋白5AC(MUC5AC)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA756Ra02 粘蛋白5AC(MUC5AC)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA756Ra01 粘蛋白5AC(MUC5AC)多克隆抗體 WB; IHC; ICC; IP.
PAA756Ra02 粘蛋白5AC(MUC5AC)多克隆抗體 WB; IHC; ICC; IP.
LAA756Ra71 粘蛋白5AC(MUC5AC)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA756Ra21 粘蛋白5AC(MUC5AC)單克隆抗體 WB; IHC; ICC; IP.
SEA756Ra 粘蛋白5AC(MUC5AC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA756Ra 粘蛋白5AC(MUC5AC)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA756Ra 粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Annals of surgical oncology Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. [PubMed: 20717734]
Investigative Ophthalmology & Visual Science Entrapment of Conjunctival Goblet Cells by Desiccation-Induced Cornification [PubMed: 21421863]
Cell & Molecular Biology Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice [PubMed: 20550619]
Mucosal Immunology Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13 [Nature: 201082a]
European Journal of Pharmacology Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways [ScienceDirect: S0014299912005523]
PLoS One Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905]
Respiratory Researh Pyocyanin-induced mucin production is associated with redox modification of FOXA2 [PubMed: PMC3765780]
Surgery A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC) [Pubmed: 24468034]
Biochemical and Biophysical Research Communications The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary disease [Pubmed:24291709]
Journal of Proteomics Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interaction [Pubmed:24726482]
Experimental Lung Research The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathway [Pubmed:24901072]
Surgery.? Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance [Pubmed:25151557]
Allergology International Leukotriene E 4 induces MUC5AC release from human airway epithelial NCI-H292 cells [Pubmed:25838093]
Respirology Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851]
S Isabela Casagrande Jeremias [Teses: Disponiveis]
American Thoracic Society International Conference Abstracts Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness [doi:10.1164]
Deletion Polymorphism and Chronic Obstructive Pulmonary Disease ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells [Pubmed:27075970]
Int J Clin Exp Med The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai [files:ijcem0020163.pdf]
Ann Am Thorac Soc Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513]
Sci Rep Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers [Pubmed:27297822]
Cytokine. Interleukin-33?induces?mucin?gene?expression?and?goblet?cell?hyperplasia?in?human?nasalepithelial?cells. [pubmed:27776277]
J Virol.? Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective. [pubmed:28275196]
Respiratory Physiology & Neurobiology Peroxiredoxin 6 suppresses Muc5ac overproduction in LPS-induced airway inflammation through H2O2-EGFR-MAPK signaling pathway. [pubmed:27884794]
Journal of Immunology Research Beneficial effects of prebiotic Saccharomyces cerevisiae mannan on allergic asthma mouse models [pubmed:28835901]
BASIC INVESTIGATION Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases [pubmed:28644234]
International Immunopharmacology Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathway [pubmed:28550735]
Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой [10.20538/1682-0363-2017-2-146-158]
Cellular Microbiology An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489]
Respiratory Research IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cells [pubmed:28931396]
Chemotherapy Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells. [pubmed:28578323]
Allergy Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps [Pubmed:29802623]
European?Journal of Pharmacology? Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling [Pubmed:29382534]
Inflammation Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in?… [Pubmed:29349683]
Pulmonary Pharmacology and Therapeutics In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extracts [Pubmed:29698781]
European Respiratory Society Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretion [Pubmed:29946002]
Clinical?and?Experimental?Ophthalmology Development of a highly sensitive and reliable enzyme-linked immunosorbent assay for MUC5aC in human tears extracted from schirmer strips [Pubmed: 30214141]
international journal of biological macromolecules Increased expression of long non-coding RNA SNHG16 correlates with tumor progression and poor prognosis in non-small cell lung cancer [Pubmed: 30287374]
Journal of Investigative?Medicine The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494]
Phytomedicine Regulation effects of naringin on diesel particulate matter-induced abnormal airway surface liquid secretion [Pubmed: 31301536]
Scientific Reports Regional comparison of Goblet cell number and Area in exposed and covered Dry eyes and their Correlation with Tear MUC5AC [Pubmed: 32076085]
Eur J Pharmacol TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK?… [Pubmed: 33068589]
journal of ethnopharmacology Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K … [Pubmed: 32344236]
Less Airway Inflammation and Goblet Cell Metaplasia in an IL-33-Induced Asthma Model of Leptin-Deficient Obese Mice []
Exp Mol Pathol The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal??- [33285209]
FEBS Open Bio Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells [33332733]
Cell Rep Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs [33535050]
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