血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Serum Amyloid A (SAA) ,etc. by FLIA (Flow Luminescence Immunoassay)
SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein
（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）
- 編號(hào)LMA885Bo
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3.5h
- 檢測(cè)范圍0.39-400ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.13 ng/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
- 價(jià)格 ¥ 2830 ¥ 2939 ¥ 3102 ¥ 3320 ¥ 3537 ¥ 3864 ¥ 4354 ¥ 5442 添加到價(jià)格計(jì)算器 計(jì)算器
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 80-97 | 90 |
| sodium citrate plasma(n=5) | 99-105 | 102 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-102% | 89-97% | 93-105% | 96-103% |
| EDTA plasma(n=5) | 94-101% | 85-93% | 86-105% | 79-94% |
| heparin plasma(n=5) | 85-104% | 83-95% | 93-101% | 99-105% |
| sodium citrate plasma(n=5) | 88-95% | 98-105% | 80-101% | 80-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA885Bo01 | 血清淀粉樣蛋白A(SAA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA885Bo01 | 血清淀粉樣蛋白A(SAA)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA885Bo21 | 血清淀粉樣蛋白A(SAA)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA885Bo | 血清淀粉樣蛋白A(SAA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA885Bo | 血清淀粉樣蛋白A(SAA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Nitric Oxide. | Effect of feeding a high fat diet on hydrogen sulfide (H 2 S) metabolism in the mouse [Pubmed:24637018] |
| Open Biochem J. | Acute Phase Response: Implication in ST-segment Elevation Myocardial Infarction [Pubmed:24894970] |
| Iran J Pharm Res. | Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical Trial [Pubmed:25237354] |
| US National Library of Medicine National Institutes of Health | No association between vitamin D levels and inflammation markers in patients with acute coronary syndrome [Pubmed:25615956] |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
| BOSCA | EFFECT OF UTERINE IMMUNOMODULATION ON SERUM AMYLOID-A CONCENTRATION AND CONCEPTION RATE IN CYCLIC NON BREEDING COWS [Tcrjournals:Source] |
| Life Sciences | Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet [PubMed: 26188592] |
| Endocrine | Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitus [PubMed: 26049370] |
| J Assist Reprod Genet. | The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndrome [PubMed: 26463878] |
| International Journal of Clinical and Experimental Medicine | Circulating serum amyloid A: a potential biomarker for infection after aneurysmal subarachnoid hemorrhage [files:ijcem0021201] |
| PLoS One. | An Anti-β-Amyloid Vaccine for Treating Cognitive Deficits in a Mouse Model of Down Syndrome [Pubmed:27023444] |
| United States Patent Application | PATHWAY SPECIFIC MARKERS FOR DIAGNOSING IRRITABLE BOWEL SYNDROME [y2016:0139148.html] |
| Veterinary World | Acute phase response in lame crossbred dairy cattle [pubmed:27956769] |
| Nature Communications | A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense [Pubmed:29410422] |
| Journal of?Animal?Sciences | Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations [Pubmed:30060086] |
| Journal?of?Animal?Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood?… [Pubmed: 30184125] |
| Journal of?Dairy?Science | Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows [Pubmed: 17430930] |
| European Review for Medical and Pharmacological Sciences | The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341] |
| Research in Veterinary Science | Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows [] |
| Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465] |
| PLoS One | Effects of vaccination on acute-phase protein response in broiler chicken [Pubmed: 32191767] |
| VETERINARY PATHOLOGY | AA Amyloid Deposition in the Central and Peripheral Nervous Systems in Flamingos [Pubmed: 32677873] |
| Comp Immunol Microbiol Infect Dis | Serum amyloid A as an marker of cow? s mastitis caused by Streptococcus sp. [Pubmed: 32505957] |
| Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy?… [] |
| РОЛЬ СЫВОРОТОЧНОГО АМИЛОИДА А И С-РЕАКТИВНОГО БЕЛКА В ПРОГНОЗИРОВАНИИ ПОСЛЕОПЕРАЦИОННЫХ ОСЛОЖНЕНИЙ И?… [] | |
| JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient??- [33474564] |
| Animals | Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid [34070499] |
| peerj | Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats [33510972] |
| Arquivo Brasileiro de Medicina Veterin¨￠ria e Zootecnia | CCS e amiloide A do leite de quartos mam¨￠rios tratados na secagem com plasma rico em plaquetas aut¨?logo, associado ou n?o a antibi¨?tico [] |
| Probiotics Antimicrob Proteins | The Effect of Feed Supplementation with EM Bokashi? Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators?… [34596883] |
| Vet Anim Sci | Effects of dietary turmeric (Curcuma longa) on innate and acquired immune responses in broiler chicken [34765801] |
| Brain Sci | Mahanimbine Improved Aging-Related Memory Deficits in Mice through Enhanced Cholinergic Transmission and Suppressed Oxidative Stress, Amyloid Levels, and?… [Pubmed:35053756] |
| J Clin Med | A Three-Protein Panel to Support the Diagnosis of Sepsis in Children [Pubmed:35329889] |
| Diabetes Metabolic Syndrome and Obesity-Targets and Therapy | Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in India [Pubmed:35717896] |
| Clinical and Experimental Immunology | Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients [Pubmed:35348620] |
| ?neklerde Subklinik Mastitis Tan?s?nda Akut Faz Proteinlerinden Amiloid A ve C-Reaktif Protein Düzeylerinin De?erlendirilmesi [] |
