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Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD282; TIL4; Toll/interleukin-1 receptor-like protein 4

（注：單次混測多因子不超過8個指標 ）

  • Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 94-102 97
EDTA plasma(n=5) 82-101 96
heparin plasma(n=5) 92-101 96
sodium citrate plasma(n=5) 91-102 95

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 78-89% 96-103% 83-101% 81-104%
EDTA plasma(n=5) 97-104% 96-103% 91-98% 80-99%
heparin plasma(n=5) 78-91% 86-103% 82-97% 78-94%
sodium citrate plasma(n=5) 80-104% 94-105% 93-101% 94-101%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的Toll樣受體2(TLR2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

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編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
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參考文獻

雜志 參考文獻
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PLoS One Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905]
Clinical and Developmental Immunology TLR2 in Pleural Fluid Is Modulated by Talc Particles during Pleurodesis [PubMed: PMC3529880]
Advances in Virology The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation [Pubmed: 24187552]
J Matern Fetal Neonatal Med. Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes [Pubmed:25028801]
Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination [Pubmed:25248345]
Iran J Immunol. Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries. [Pubmed:25265998]
Int J Dermatol. Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapy [Pubmed:25266302]
BMC Immunology The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases [Pubmed:25406630]
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