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轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Transforming Growth Factor Beta 1 (TGFb1) ,etc. by FLIA (Flow Luminescence Immunoassay)

TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

(注:單次混測多因子不超過8個指標 )

  • 轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • 轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 80-94 90
EDTA plasma(n=5) 89-103 93
heparin plasma(n=5) 78-94 81

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 99-105% 78-98% 98-105%
EDTA plasma(n=5) 79-102% 82-98% 80-95% 79-93%
heparin plasma(n=5) 78-89% 86-101% 97-105% 91-105%

穩(wěn)定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠)標準品或樣本100μL及磁珠10μL,
    37°C酶標板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠,其中的轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合,然后加入生物素化的轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體,將未結合的生物素化抗體洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
APA124Mu62 轉化生長因子β1(TGFb1)活性蛋白 Cell?culture;?Activity?Assays.
RPA124Mu02 轉化生長因子β1(TGFb1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA124Mu01 轉化生長因子β1(TGFb1)活性蛋白 Cell?culture;?Activity?Assays.
RPA124Mu01 轉化生長因子β1(TGFb1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA124Mu61 轉化生長因子β1(TGFb1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA124Mu01 轉化生長因子β1(TGFb1)多克隆抗體 WB; IHC; ICC; IP.
PAA124Mu02 轉化生長因子β1(TGFb1)多克隆抗體 WB; IHC; ICC; IP.
LAA124Mu81 轉化生長因子β1(TGFb1)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
LAA124Mu71 轉化生長因子β1(TGFb1)多克隆抗體(生物素標記) WB; IHC; ICC.
SEA124Mu 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA124Mu 轉化生長因子β1(TGFb1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA124Mu 轉化生長因子β1(TGFb1)檢測試劑盒(化學發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA124Mu 轉化生長因子β1(TGFb1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA124Mu01 轉化生長因子β1(TGFb1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells [Pubmed: 32235811]
Journal of Oral Biology and Craniofacial Research Levels of TGF-β1 in peri-miniscrew implant crevicular fluid [Pubmed: 32211284]
Acta Histochem Paeoniflorin accelerates foot wound healing in diabetic rats though activating the Nrf2 pathway [Pubmed: 33166863]
EUROPEAN JOURNAL OF PHARMACOLOGY The anti-asthmatic potential of flavonol kaempferol in an experimental model of allergic airway inflammation [Pubmed: 33122672]
Journal of Cosmetic Dermatology Examination of androgenetic alopecia with serum biomarkers [Pubmed: 32969583]
MOLECULAR AND CELLULAR BIOCHEMISTRY Soluble recombinant human thrombomodulin suppresses inflammation-induced gastrointestinal tumor growth in a murine peritonitis model [Pubmed: 32767229]
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY Hepatoprotective effect of Linagliptin against liver fibrosis induced by carbon tetrachloride in mice [Pubmed: 32726558]
EXPERIMENTAL DERMATOLOGY Decreased suppression of CD8+ and CD4+ T cells by peripheral Regulatory T cells in Generalized Vitiligo due to reduced NFATC1 and FOXP3 proteins [Pubmed: 32682346]
Scientific Reports Erlotinib can halt adenine induced nephrotoxicity in mice through modulating ERK1/2, STAT3, p53 and apoptotic pathways [Pubmed: 32661331]
international journal of biological macromolecules Chlorella vulgaris α-L-arabino-α-L-rhamno-α, β-D-galactan structure and mechanisms of its anti-inflammatory and anti-remodelling effects [Pubmed: 32565301]
Exp Cell Res Exosomal miR-532–5p from bone marrow mesenchymal stem cells reduce intervertebral disc degeneration by targeting RASSF5 [Pubmed: 32464126]
JOURNAL OF IMMUNOLOGY RESEARCH The Sufficient Immunoregulatory Effect of Autologous Bone Marrow-Derived Mesenchymal Stem Cell Transplantation on Regulatory T Cells in Patients with … [Pubmed: 32411794]
Frontiers in Pharmacology M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice [Pubmed: 33041798]
Food & Function Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial?… []
Effect of metformin and indole-3-carbinol on a rat model of Parkinson's disease induced by 6-hydroxydopamine []
Research square Vitamin D Status in Dupuytren's Disease: Association with Clinical Status and Vitamin D Receptor Expression []
Phytomedicine Menthacarin attenuates experimental colitis []
Immunomodulatory effects of sCD83 via induction of TGF-β1 and IDO: an in vitro and rat orthotopic liver transplantation study []
BMC Complement Med Ther Suppression of autophagy through JAK2/STAT3 contributes to the therapeutic action of rhynchophylline on asthma [33413331]
Life Sci Celecoxib ameliorates liver cirrhosis via reducing inflammation and oxidative stress along spleen-liver axis in rats [33577848]
CLINICAL SCIENCE Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes [33491733]
Bioact Mater New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739]
Acta Biomater Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473]
Mol Cell Biochem Human umbilical cord-derived mesenchymal stem cells and their chondroprogenitor derivatives reduced pain and inflammation signaling and promote regeneration in??- [33864569]
Cell Death & Disease Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization [34006822]
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928]
Vascular pharmacology Nectin-4 promotes lymphangiogenesis and lymphatic metastasis in breast cancer by regulating CXCR4-LYVE1 axis [33945869]
Equine Vet J Limited added value of negative pressure wound therapy compared to calcium alginate dressings for second intention healing in a non?\contaminated and??- [34115409]
Stem Cells International The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin??- [33381190]
Journal of Pharmacy and Pharmacology Pharmacodynamic evaluation of dihydroxyflavone derivate chrysin in a guinea pig model of allergic asthma [33793800]
Bull. Egypt. Soc. Physiol. Sci. Flaxseed Oil Supplementation Afford Comparable Therapeutic effect to Metformin in Bleomycin Induced Pulmonary Fibrosis Rat model []
D£?ˉ Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model []
Bioactive Materials Calcium phosphate-based materials regulate osteoclast-mediated osseointegration []
Mediators of Inflammation Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of |á1, 3 ?- []
Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction []
Andrology X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model [34236146]
Aging Yiqi Jiemin decoction alleviates allergic rhinitis in a guinea pig model by suppressing inflammation, restoring Th1/Th2 balance, and improving cellular?… [34315133]
Tissue Eng Regen Med Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method [34339025]
Am J Transl Res Wuling San and Xiao Chaihu Decoction affect airway inflammatory response and airway smooth muscle cell proliferation in mice with allergic asthma via miR?… [34786062]
Biomacromolecules In Situ Forming Cellulose Nanofibril-Reinforced Hyaluronic Acid Hydrogel for Cartilage Regeneration [34723499]
Brain Sci Neuroprotective Effect of Clobenpropit against Lipopolysaccharide-Induced Cognitive Deficits via Attenuating Neuroinflammation and Enhancing Mitochondrial?… [34942919]
Biomedicines Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes [34829910]
J Inflamm Res Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice [Pubmed:35058704]
Cancer Commun (Lond) Anti‐PD‐L1/TGF‐βR fusion protein (SHR‐1701) overcomes disrupted lymphocyte recovery‐induced resistance to PD‐1/PD‐L1 inhibitors in lung cancer [Pubmed:34981670]
Polymers (Basel) 3D Printed Scaffold Based on Type I Collagen/PLGA_TGF-β1 Nanoparticles Mimicking the Growth Factor Footprint of Human Bone Tissue [Pubmed:35267680]
Biomed Pharmacother Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis [Pubmed:35453007]
Biomed Pharmacother Huangbai liniment and berberine promoted wound healing in high-fat diet/Streptozotocin-induced diabetic rats [Pubmed:35430394]
Antioxidants Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory?… [Pubmed:35204215]
Frontiers in Molecular Neuroscience Red Nucleus Interleukin-6 Evokes Tactile Allodynia in Male Rats Through Modulating Spinal Pro-inflammatory and Anti-inflammatory Cytokines [Pubmed:35465093]
Vaccines Age-Dependent Dynamics of Maternally Derived Antibodies (MDAs) and Understanding MDA-Mediated Immune Tolerance in Foot-and-Mouth Disease-Vaccinated?… [Pubmed:35632433]
Biomaterials Advances Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats []
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