內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay)
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號LMA638Gu
- 物種Cavia (Guinea pig ，豚鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.1-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.033 ng/mL.
- 樣本類型Serum, plasma and other biological fluids.
- 下載 英文說明書 中文說明書
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 83-93 | 90 |
| EDTA plasma(n=5) | 79-98 | 83 |
| heparin plasma(n=5) | 82-94 | 89 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-101% | 87-96% | 94-101% | 87-96% |
| EDTA plasma(n=5) | 80-90% | 92-101% | 94-102% | 78-91% |
| heparin plasma(n=5) | 98-105% | 82-103% | 83-101% | 80-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Cavia (Guinea pig ，豚鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA638Gu01 | 內(nèi)脂素(VF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA638Gu01 | 內(nèi)脂素(VF)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA638Gu21 | 內(nèi)脂素(VF)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA638Gu | 內(nèi)脂素(VF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA638Gu | 內(nèi)脂素(VF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| J Physiol Pharmacol. | Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat. [Pubmed: 24304571] |
| Endocr J. | CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658] |
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| Eur J Cancer Prev. | Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization. [Pubmed:25055182] |
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| Bioscience Reports | Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats [Pubmed:29784871] |
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| Nutrire | Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children [10.1186:s41110-018-0060-7] |
| DNA?and?Cell?Biology | Transcriptional Regulation of?NAMPT?Gene by Glycogen Synthase Kinase 3β in Goat Adipocytes [Pubmed: 30615481] |
| Jundishapur?Journal of?Chronic?Disease?Care | The Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men [] |
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| EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY | Different Ratios of Corn and Coconut Oil Blends in High?\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats [] |
