碳酸酐酶Ⅳ(CA4)活性蛋白
Active Carbonic Anhydrase IV (CA4)
CA-IV; CAIV; Car4; RP17; Retinitis Pigmentosa 17(Autosomal Dominant); Carbonate dehydratase IV
- 編號APD071Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點6.5
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2? H2O = HCO3-? H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA4 is a GPI anchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO2?taste sensor. The activity of recombinant human CA4 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA4 (diluted by assay buffer) to 50 μL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.
One unit of enzyme activity is defined as the 1 μg of enzyme required to convert 1 pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant human CA4 is > 15 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APD071Hu01 | 碳酸酐酶Ⅳ(CA4)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPD071Hu01 | 碳酸酐酶Ⅳ(CA4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD071Hu01 | 碳酸酐酶Ⅳ(CA4)多克隆抗體 | WB; IHC; ICC; IP. |
| LAD071Hu71 | 碳酸酐酶Ⅳ(CA4)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAD071Hu81 | 碳酸酐酶Ⅳ(CA4)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAD071Hu21 | 碳酸酐酶Ⅳ(CA4)單克隆抗體 | WB; IHC; ICC/IF |
| MAD071Hu25 | 碳酸酐酶Ⅳ(CA4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD071Hu22 | 碳酸酐酶Ⅳ(CA4)單克隆抗體 | WB |
| MAD071Hu23 | 碳酸酐酶Ⅳ(CA4)單克隆抗體 | WB; IHC; ICC; IP. |
| SED071Hu | 碳酸酐酶Ⅳ(CA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD071Hu | 碳酸酐酶Ⅳ(CA4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSD071Hu01 | 碳酸酐酶Ⅳ(CA4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
