羧酸酯酶1(CES1)活性蛋白
Active Carboxylesterase 1 (CES1)
ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1
- 編號(hào)APC374Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5( Assay Buffer), initiated by addition 50 μL of various concentrations of CES1 (dilute by Assay Buffer) to 50 μL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.
One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant human CES1 is 600 pmol/min/μg.
Specific Activity (pmol/min/μg)=
△OD=Adjusted for Substrate Blank
F=Conversion Factor(convert from standard curve of 4-Nitrophenol)
T= Time
N=Amount of enzyme
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPC374Hu61 | 羧酸酯酶1(CES1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC374Hu01 | 羧酸酯酶1(CES1)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC374Hu01 | 羧酸酯酶1(CES1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC374Hu61 | 羧酸酯酶1(CES1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAC374Hu01 | 羧酸酯酶1(CES1)多克隆抗體 | WB; IHC; ICC; IP. |
| SEC374Hu | 羧酸酯酶1(CES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC374Hu | 羧酸酯酶1(CES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Nanomedicine | Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug Interactions [Pubmed: 32764932] |
