CD2分子(CD2)活性蛋白
Active Cluster Of Differentiation 2 (CD2)
LFA2; SRBC; T11; T-Cell Surface Antigen T11/Leu-5; LFA-3 Receptor; Lymphocyte Function Associated Antigen 2; Erythrocyte Receptor; Rosette Receptor; Sheep Red Blood Cell Receptor
- 編號(hào)APB576Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)8.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1440 ¥ 3600 ¥ 7200 ¥ 21600 ¥ 54000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Cluster of Differentiation 2 (CD2) is a surface antigen found on all peripheral blood T-cells. CD2 functions as an activator of T lymphocytes and thymocytes. Mature human CD2 consists of a 185 amino acid (aa) extracellular domain (ECD) with an Ig-like V-type and an Ig-like C2 type domain, a 26 aa transmembrane segment, and a 116 aa cytoplasmic tail. Cluster of Differentiation 5 (CD5) has been identified as an interactor of CD2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD2 and recombinant human CD5. Briefly, CD2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CD2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human CD2 and recombinant human CD5 was shown in Figure 1, the EC50 was 1.88 ug/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPB576Hu61 | CD2分子(CD2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB576Hu01 | CD2分子(CD2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB576Hu61 | CD2分子(CD2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RAB576Hu21 | CD2分子(CD2)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAB576Hu21 | CD2分子(CD2)單克隆抗體 | WB; IHC; ICC; IP. |
| FAB576Hu02 | 抗CD2分子(CD2)單克隆抗體 | FCM |
| FAB576Hu04 | 抗CD2分子(CD2)單克隆抗體 | FCM |
| FAB576Hu06 | 抗CD2分子(CD2)單克隆抗體 | FCM |
