細(xì)胞色素P450家族成員1A2(CYP1A2)活性蛋白
Active Cytochrome P450 1A2 (CYP1A2)
CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2
- 編號(hào)APD294Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)8.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 2280 ¥ 5700 ¥ 11400 ¥ 34200 ¥ 85500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Gene sequencing
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant CYP1A2, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
CYP1A2 (Cytochrome P450 1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Besides, ASAH1 (Acid ceramidase) has been identified as an interactor of CYP1A2 through affinity capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ASAH1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1A2 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1A2 and ASAH1 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPD294Hu01 | 細(xì)胞色素P450家族成員1A2(CYP1A2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD294Hu01 | 細(xì)胞色素P450家族成員1A2(CYP1A2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPD294Hu02 | 細(xì)胞色素P450家族成員1A2(CYP1A2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPD294Hu21 | 細(xì)胞色素P450家族成員1A2(CYP1A2)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAD294Hu01 | 細(xì)胞色素P450家族成員1A2(CYP1A2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD294Hu02 | 細(xì)胞色素P450家族成員1A2(CYP1A2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD294Hu08 | 細(xì)胞色素P450家族成員1A2(CYP1A2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAD294Hu71 | 細(xì)胞色素P450家族成員1A2(CYP1A2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SED294Hu | 細(xì)胞色素P450家族成員1A2(CYP1A2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD294Hu | 細(xì)胞色素P450家族成員1A2(CYP1A2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Environmental Monitoring and Assessment | Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytes [Pubmed:25471622] |
| The Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4 [PubMed: 25733458] |
| Molecular Nutrition Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats [Pubmed:27133590] |
| Molecular Nutrition &?Food?Research | Phenethyl isothiocyanate?and?indole-3-carbinol?from?cruciferous?vegetables, but not furanocoumarins from apiaceous?vegetables, reduced PhIP-induced DNA adducts in Wistar rats. [pubmed:27133590] |
| ?Journal of FisheriesSciences. com | Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stress [b0407ebde042c4f61b728bc11a586e6c] |
| Toxicology | Liver lobe and strain differences in the activity of murine cytochrome p450 enzymes [Pubmed:29879457] |
| Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus [] |
