二胺氧化酶(ABP1)活性蛋白
Active Amiloride Binding Protein 1 (ABP1)
AOC1; DAO; KAO; Histaminase; Kidney amine oxidase; Diamine Oxidase; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1
- 編號APA656Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1805 ¥ 4512 ¥ 9024 ¥ 27072 ¥ 67680
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Amiloride binding protein (ABP1), also known as amine oxidase copper domain-containing protein 1 (AOC1), is an approximately 110 kDa member of the copper-containing amine oxidase family. ABP1 is a glycosylated enzyme that forms disulfide-linked homodimers. It is stored in cytoplasmic granules of epithelial cells in the kidney, placenta, uterus, lung, and intestine, and its extracellular release can be induced by heparin. ABP1 exhibits a substrate preference for histamine and putrescine, generating hydrogen peroxide and ammonia in an oxidative deamination reaction. ABP1 activity plays an important role in the catabolism of histamine and other bioactive polyamines. The activity of recombinant human ABP1 was measured by its ability to produce hydrogen peroxide during the oxidation of histamine. The reaction was performed in 50 mM HEPES, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of ABP1 (diluted by assay buffer) to 50 μl substrate mixture of 10 uM histamine, 2 units/mL HRP and 100 μM AUR. Read at excitation and emission wavelengths of 544 nm and 590 nm (top read), respectively in kinetic mode for 5 minutes.
用法
Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA656Hu61 | 二胺氧化酶(ABP1)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA656Hu61 | 二胺氧化酶(ABP1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA656Hu01 | 二胺氧化酶(ABP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA656Hu02 | 二胺氧化酶(ABP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA656Hu01 | 二胺氧化酶(ABP1)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| PAA656Hu02 | 二胺氧化酶(ABP1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA656Hu71 | 二胺氧化酶(ABP1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA656Hu22 | 二胺氧化酶(ABP1)單克隆抗體 | WB,IHC |
| MAA656Hu21 | 二胺氧化酶(ABP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA656Hu25 | 二胺氧化酶(ABP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA656Hu27 | 二胺氧化酶(ABP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA656Hu29 | 二胺氧化酶(ABP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA656Hu23 | 二胺氧化酶(ABP1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA656Hu24 | 二胺氧化酶(ABP1)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA656Hu02 | 抗二胺氧化酶(ABP1)單克隆抗體 | Flow cytometry. |
| FAA656Hu04 | 抗二胺氧化酶(ABP1)單克隆抗體 | FCM |
| FAA656Hu06 | 抗二胺氧化酶(ABP1)單克隆抗體 | FCM |
| FAA656Hu08 | 抗二胺氧化酶(ABP1)單克隆抗體 | FCM |
| SEA656Hu | 二胺氧化酶(ABP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA656Hu | 二胺氧化酶(ABP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| ?Biochimica et Biophysica Acta (BBA)-General Subjects | Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485] |
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| Advances in Experimental Medicine and Biology | Serum Diamine Oxidase in Pseudoallergy in the Pediatric Population [pubmed:28804811] |
| PLoS One | Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats [pubmed:28622357] |
| Nutrients | Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron [Pubmed:29861488] |
| Scientific Reports | Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsia [Pubmed:29679053] |
| British Journal of Nutrition | Postoperative symbiotic in patients with head and neck cancer: a double-blind randomised trial [Pubmed:29277158] |
| Food?&?Function | The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer [Pubmed:29384543] |
| 碩士論文 | LOCALIZATION OF COPPER-CONTAINING AMINE OXIDASE ENZYMES IN THE BLOOD [] |
| International?Journal?of?Clinical?and?Experimental?Medicine | Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease [] |
| Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease [] |
| Acta Diabetologica | Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated mice [Pubmed: 31749050] |
| Biomed Research International | Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099] |
| FASEB JOURNAL | HMGB1‐associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in rats [Pubmed: 31961020] |
| JOURNAL OF ETHNOPHARMACOLOGY | The effect of Poria cocos ethanol extract on the intestinal barrier function and intestinal microbiota in mice with breast cancer [Pubmed: 33039631] |
| Cell Death Discovery | Fecal microbiota transplantation from patients with autoimmune encephalitis modulates Th17 response and relevant behaviors in mice [Pubmed: 32821439] |
| The Journal of Nutritional Biochemistry | Effects of fucoidan on tumor prevention and gut flora [] |
| Chemical Biology & Dug Design | Design and development of novel fasudil derivatives as potent anti‐breast cancer agent that improves intestinal flora and intestinal barrier function in rats [34587363] |
| Journal of Functional Foods | Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways [] |
| Stem Cell Research & Therapy | Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model [] |
