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半乳糖凝集素3(GAL3)活性蛋白

Active Galectin 3 (GAL3)

LGALS3; CBP35; GALBP; GALIG; MAC2; 35 kDa lectin; Carbohydrate-binding protein 35; Galactose-specific lectin 3; Galactoside-binding protein; Laminin-binding protein; Lectin L-29

  • 半乳糖凝集素3(GAL3)活性蛋白 產(chǎn)品包裝(模擬)
  • 半乳糖凝集素3(GAL3)活性蛋白 產(chǎn)品包裝(模擬)
  • APA303Ra01.png Figure. SDS-PAGE
  • 半乳糖凝集素3(GAL3)活性蛋白 Figure. Western Blot
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Galectin 3 (GAL3) is a member of the lectin family, of which 14 mammalian galectins have been identified. It is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. The protein also has been demonstrated to be involved in cancer, inflammation and fibrosis, heart disease, and stroke. GAL3 is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. It also can agglutinate red blood. In this case, we choose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (HA) is as follows, two-fold dilute the recombinant rat GAL3 with 0.01M PBS (pH7.4), add 50μL a serial dilution of GAL3 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL3, replace with 50μL 0.01M PBS. Then add 50μL 1% RaE to each well and mixed gently. The plate is incubated for 1-2 hours at room temperature. The results are shown in Figure 1. The minimal effective concentration of GAL3 is 2.5μg/mL. (A) 1% RaE treated with 2.5μg/mL GAL3 for 2h; (B) Negative control without GAL3.
Figure. The hemagglutination activity of recombinant rat GAL3.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Rattus norvegicus (Rat，大鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA303Ra01 半乳糖凝集素3(GAL3)活性蛋白 Cell?culture;?Activity?Assays.
RPA303Ra01 半乳糖凝集素3(GAL3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA303Ra01 半乳糖凝集素3(GAL3)多克隆抗體 WB; IHC; ICC; IP.
FAA303Ra01 抗半乳糖凝集素3(GAL3)多克隆抗體 Flow cytometry.
LAA303Ra81 半乳糖凝集素3(GAL3)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAA303Ra71 半乳糖凝集素3(GAL3)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA303Ra24 半乳糖凝集素3(GAL3)單克隆抗體 WB; IHC; ICC; IP.
MAA303Ra23 半乳糖凝集素3(GAL3)單克隆抗體 WB; IHC; ICC; IP.
MAA303Ra21 半乳糖凝集素3(GAL3)單克隆抗體 WB; IHC; ICC; IP.
MAA303Ra22 半乳糖凝集素3(GAL3)單克隆抗體 WB; IHC; ICC; IP.
FAA303Ra04 抗半乳糖凝集素3(GAL3)單克隆抗體 Flow cytometry.
FAA303Ra06 抗半乳糖凝集素3(GAL3)單克隆抗體 Flow cytometry.
FAA303Ra02 抗半乳糖凝集素3(GAL3)單克隆抗體 Flow cytometry.
SEA303Ra 半乳糖凝集素3(GAL3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA303Ra 半乳糖凝集素3(GAL3)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Clinica Chimica Acta Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population [Pubmed:25578395]
Scientific Reports Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension [PubMed: 25747153]
Int J Mol Sci The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension [PubMed: 25984599]
Scientifi Reportcs Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices [PubMed: 26582585]
Clin Chim Acta Galectin-1 and galectin-4: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population [PubMed: 25578396]
JOURNAL OF THE AMERICAN SOCIETY OF HYPERTENSION Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4. [pubmed:28431936]
昆明醫(yī)科大學(xué)學(xué)報(bào) 半乳糖凝集素3 在COPD 和肺癌中的表達(dá)及其意義. [2095610X]
Archives of Medical Science Can we define migraine patients with blood high-sensitivity C-reactive protein and galectin-3 levels in the emergency department? [publication:305625095]
Biosensors & Bioelectronics A sensitive sandwich-type immunosensor for the detection of galectin-3 based on N-GNRs-Fe-MOFs@AuNPs nanocomposites and a novel AuPt-methylene blue nanorod. [pubmed:29096363]
Scientific Reports The profile of selected single nucleotide polymorphisms in patients with hypertension and heart failure with preserved and mid-range ejection fraction. [pubmed:28827564]
Clinica Chimica Acta Galectin-3 in patients with coronary heart disease and atrial fibrillation [Pubmed:29287900]
Advances in Experimental?Medicine?and?Biology Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028]
FASEB?Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922]
american journal of physiology-heart and circulatory physiology Macrophage galectin-3 enhances intimal translocation of vascular calcification in diabetes mellitus [Pubmed: 32216615]
Cardiology Galectin-3 Induces Atrial Fibrosis by Activating the TGF-β1/Smad Pathway in Patients with Atrial Fibrillation [Pubmed: 32516780]
AUSTRALIAN DENTAL JOURNAL The effect of initial periodontal treatment on gingival crevicular fluid galectin?\3 levels in participants with periodontal disease [33378559]
Cell Death & Disease Trajectory modeling of endothelial-to-mesenchymal transition reveals galectin-3 as a mediator in pulmonary fibrosis [33771973]
Int J Clin Pract Serum Galectin?\3 Levels in Patients with Psoriasis [34137138]
Phytomedicine Zerumbone, a humulane sesquiterpene from Syringa pinnatifolia, attenuates cardiac fibrosis by inhibiting of the TGF-β1/Smad signaling pathway after myocardial?… [Pubmed:35405613]
Medicine Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?… [Pubmed:35687771]
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