谷胱甘肽S轉(zhuǎn)移酶μ1(GSTM1)活性蛋白
Active Glutathione S Transferase Mu 1 (GSTM1)
GST-M1; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; MU1; GST HB subunit 4
- 編號APA658Ga01
- 物種Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點7.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. GSTM1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 μl 200 mM glutathione (reduced) and 10 μl 100 mM CDNB in 980 μl 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 μl mixed substrates to 50 μl different concentrations of recombinant chicken GSTM1, mix gently. Incubated at 37℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant chicken GSTM1 is >2500 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Chicken (Gallus，雞) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA658Ga01 | 谷胱甘肽S轉(zhuǎn)移酶μ1(GSTM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA658Ga01 | 谷胱甘肽S轉(zhuǎn)移酶μ1(GSTM1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA658Ga01 | 谷胱甘肽S轉(zhuǎn)移酶μ1(GSTM1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA658Ga71 | 谷胱甘肽S轉(zhuǎn)移酶μ1(GSTM1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA658Ga81 | 谷胱甘肽S轉(zhuǎn)移酶μ1(GSTM1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
參考文獻
| 雜志 | 參考文獻 |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019] |
| CHEMICAL SENSES | Interactions Between Odorants and Glutathione Transferases in the Human Olfactory Cleft [Pubmed: 32822470] |
