Gremlin 1蛋白(GREM1)活性蛋白
Active Gremlin 1 (GREM1)
PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1
- 編號APC128Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點9.0
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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WB圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Gremlin 1 (GREM1) is an inhibitor in the TGF-beta signaling pathway. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GREM1 and recombinant human BMP2. Briefly, GREM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BMP2-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST and incubated for 1h with anti-GREM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human GREM1 and recombinant human BMP2 was shown in Figure 1，the EC50?for this effect is 0.1~0.562 ug/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APC128Hu02 | Gremlin 1蛋白(GREM1)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC128Hu01 | Gremlin 1蛋白(GREM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC128Hu01 | Gremlin 1蛋白(GREM1)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC128Hu02 | Gremlin 1蛋白(GREM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC128Hu01 | Gremlin 1蛋白(GREM1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC128Hu02 | Gremlin 1蛋白(GREM1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAC128Hu81 | Gremlin 1蛋白(GREM1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAC128Hu71 | Gremlin 1蛋白(GREM1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAC128Hu21 | Gremlin 1蛋白(GREM1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC128Hu23 | Gremlin 1蛋白(GREM1)單克隆抗體 | WB; IHC; ICC; IP. |
| RAC128Hu21 | Gremlin 1蛋白(GREM1)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAC128Hu22 | Gremlin 1蛋白(GREM1)單克隆抗體 | WB,IHC |
| SEC128Hu | Gremlin 1蛋白(GREM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC128Hu | Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Lung | Intrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial Hypertension [PubMed: 25926293] |
| Tumour Biol | Overexpression of Gremlin promotes non-small cell lung cancer progression [PubMed: 26392110] |
| Int J Cardiol | Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patients [PubMed: 26539964] |
| International Journal of Cardiology | Triple?head-to-head?comparison?of?fibrotic?biomarkers?galectin-3,?osteopontin?and?gremlin-1?for?long-termprognosis?in?suspected?and?proven?acute?heart?failure?patients [Pubmed:26539964] |
| American Journal of Respiratory Cell and Molecular Biology | BMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAH [Pubmed:27187737] |
| J Rheumatol | GREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and Invasiveness [Pubmed:26834210] |
| European cells & materials | The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. [pubmed:28266688] |
| Sci Rep. | Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients. [pubmed:28327551] |
| American Journal of Respiratory Cell and Molecular Biology | Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial … [rcmb.2015-0402OC] |
| Molecular Medicine Reports | Gremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cells [pubmed:28260028] |
| Cellular Haemostasis and Platelets | Platelets as a novel source of Gremlin-1: Implications for thrombo inflammation [pubmed:27929199] |
| Scientific Reports | Gremlin, A Potential Urinary Biomarker of Anca-Associated Crescentic Glomerulonephritis [Pubmed: 31053735] |
| International Journal of Molecular Sciences | Transcriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage Regeneration [Pubmed: 31878307] |
| American Journal of Physiology-Cell Physiology | Suppression of microRNA-23a/b-3p delays the development degenerative joint disease by activating Grem1 and TGF-β/Smad signaling pathway [Pubmed: 32186934] |
| Inflammopharmacology | Protective role of microRNA-23a/b-3p inhibition against osteoarthritis through Gremlin1-depenent activation of TGF-β/smad signaling in chondrocytesa [Pubmed:35441352] |
| Mol Carcinog | CAF‐derived exosomes transmitted Gremlin‐1 promotes cancer progression and decreases the sensitivity of hepatoma cells to sorafenib [Pubmed:35638711] |
