音猬因子(SHH)活性蛋白
Active Hedgehog Homolog, Sonic (SHH)
HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N
- 編號(hào)APB831Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)7.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1267 ¥ 3168 ¥ 6336 ¥ 19008 ¥ 47520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. It has been reported that SHH can induce alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells. To test the bioactivity of SHH, C3H10T1/2 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of SHH for 24h and alkaline phosphatase levels in the cell supernatant were determined by ELISA. Alkaline phosphatase levels in the cell supernatant of C3H10T1/2 cells increased significantly after stimulated with SHH have shown in Figure1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB831Hu01 | 音猬因子(SHH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB831Hu61 | 音猬因子(SHH)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB831Hu01 | 音猬因子(SHH)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB831Hu61 | 音猬因子(SHH)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB831Hu01 | 音猬因子(SHH)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB831Hu22 | 音猬因子(SHH)單克隆抗體 | WB; IHC |
| MAB831Hu21 | 音猬因子(SHH)單克隆抗體 | WB,IHC |
| MAB831Hu24 | 音猬因子(SHH)單克隆抗體 | WB; IHC; ICC; IP. |
| RAB831Hu21 | 音猬因子(SHH)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAB831Hu23 | 音猬因子(SHH)單克隆抗體 | IHC. |
| SEB831Hu | 音猬因子(SHH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEB831Hu | 音猬因子(SHH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB831Hu | 音猬因子(SHH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer [y2016:0077095.html] |
| Annals of the Rheumatic Diseases | Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis [pubmed:28495672] |
