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高遷移率族蛋白1(HMGB1)活性蛋白

Active High Mobility Group Protein 1 (HMGB1)

HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein

  • 高遷移率族蛋白1(HMGB1)活性蛋白 產(chǎn)品包裝(模擬)
  • 高遷移率族蛋白1(HMGB1)活性蛋白 產(chǎn)品包裝(模擬)
  • APA399Ra01.jpg Figure. SDS-PAGE
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

High Mobility Group Protein 1 (HMGB1) is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. Besides, Toll Like Receptor 2 (TLR2) has been identified as an interactor of HMGB1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat HMGB1 and recombinant human TLR2. Briefly, HMGB1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to TLR2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HMGB1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat HMGB1 and recombinant human TLR2 was shown in Figure 1, the EC50?for this effect is 0.25 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Rattus norvegicus (Rat，大鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA399Ra01 高遷移率族蛋白1(HMGB1)活性蛋白 Cell?culture;?Activity?Assays.
RPA399Ra01 高遷移率族蛋白1(HMGB1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA399Ra01 高遷移率族蛋白1(HMGB1)多克隆抗體 WB,IHC
LAA399Ra81 高遷移率族蛋白1(HMGB1)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAA399Ra71 高遷移率族蛋白1(HMGB1)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA399Ra21 高遷移率族蛋白1(HMGB1)單克隆抗體 WB; IHC; ICC; IP.
SEA399Ra 高遷移率族蛋白1(HMGB1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA399Ra 高遷移率族蛋白1(HMGB1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA399Ra01 高遷移率族蛋白1(HMGB1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志 參考文獻(xiàn)
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United States Patent Application METHOD OF MAKING A SKIN CARE COMPOSITION [y2016:0077080.html]
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Biochemical and Biophysical Research Communications Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release [pubmed:29146189]
Pulmonary Pharmacology & Therapeutics HMGB1 promotes HLF-1 proliferation and ECM production through activating HIF1-a-regulated aerobic glycolysis [pubmed:28571757]
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Frontiers in Immunology Glycyrrhizin Protects Mice against Experimental Autoimmune Encephalomyelitis by Inhibiting HMGB1 Expression and Neuronal HMGB1 Release [Pubmed:30013568]
Oncoimmunology Bromodomain inhibition exerts its therapeutic potential in malignant pleural mesothelioma by promoting immunogenic cell death and changing the tumor immune?… [Pubmed:29399399]
Cancer Immunology, Immunotherapy Tumor lysate-based vaccines: on the road to immunotherapy for gallbladder cancer [Pubmed:29600445]
Cytokine Porcine reproductive and respiratory syndrome virus induces concurrent elevation of High Mobility Group Box-1 protein and pro-inflammatory cytokines in?… [Pubmed:29895396]
Frontiers in Neurology Ursolic Acid Ameliorates Inflammation in Cerebral Ischemia and Reperfusion Injury Possibly via High Mobility Group Box 1/Toll-Like Receptor 4/NFκB?… [Pubmed:29867706]
Biomedicine & Pharmacotherapy Oridonin enhances the cytotoxicity of 5-FU in renal carcinoma cells by inducting necroptotic death [Pubmed:29958141]
Inflammation?Research Luteolin activates ERK1/2- and Ca-dependent HO-1 induction that reduces LPS-induced HMGB1, iNOS/NO, and COX-2 expression in RAW264.7 cells and?… [Pubmed:29497773]
Journal of?Food?and?Drug?Analysis Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice [Pubmed:29389558]
Biochemical and Biophysical?Research?Communications Sirt1 S-nitrosylation induces acetylation of HMGB1 in LPS-activated RAW264. 7 cells and endotoxemic mice [Pubmed:29680657]
Neurological Sciences Thrombomodulin, alarmin signaling, and copeptin: cross-talk between obesity and acute ischemic stroke initiation and severity in Egyptians [Pubmed:29637447]
International Journal of Gynecology & Obstetrics Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsia [Pubmed:29569400]
Advances in Experimental?Medicine?and?Biology Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028]
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Biomaterials Science Delivery of 5'-Triphosphate RNA with Endosomolytic Nanoparticles Potently Activates RIG-I to Improve Cancer Immunotherapy [Doi: 10.1039/C8BM01064A?]
iris-AperTO []
Toxicological Sciences Apoptosis resistance in fibroblasts precedes progressive scarring in pulmonary fibrosis and is partially mediated by Toll-like receptor 4 activation [Pubmed: 31020321]
Molecular Medicine FBXW7 suppresses HMGB1-mediated innate immune signaling to attenuate hepatic inflammation and insulin resistance in a mouse model of nonalcoholic?… [Pubmed: 31215394]
international immunopharmacology Dulaglutide mitigates inflammatory response in fibroblast-like synoviocytes [Pubmed: 31185450]
Food & Function Network pharmacology oriented study reveals inflammatory state-dependent dietary supplement hepatotoxicity responses in normal and diseased rats [Pubmed: 31140472]
International Immunopharmacology BET bromodomain inhibitor JQ1 promotes immunogenic cell death in tongue squamous cell carcinoma [Pubmed: 31600692]
Experimental and Therapeutic Medicine High mobility group box protein 1 neutralization therapy in ovine bacteremia: Lessons learned from an ovine septic shock model incorporating intensive care support [Pubmed: 31602200]
Oxidative Medicine and Cellular Longevity Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters?… [Pubmed: 31737175]
Pathobiology Magnetic hyperthermia using self-controlled heating elements consisting of Fe-Al milling alloy induces cancer cell apoptosis while preserving skeletal muscle [Pubmed: 31722347]
Canadian Journal of Physiology and Pharmacology Suppression of high-mobility group box 1 ameliorates xerostomia in a sjogren syndrome-triggered mouse model [Pubmed: 31935120]
Neurotoxicity Research HMGB1 Mediates Paraquat-Induced Neuroinflammatory Responses via Activating RAGE Signaling Pathway [Pubmed: 31858421]
Journal of Clinical Medicine Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109]
Journal of Immunology Research Dendritic Cells Loaded with Heat Shock-Conditioned Ovarian Epithelial Carcinoma Cell Lysates Elicit T Cell-Dependent Antitumor Immune Responses In Vitro [Pubmed: 31886313]
urologic oncology A novel diagnostic tool for the detection of bladder cancer: Measurement of urinary high mobility group box-1 [Pubmed: 32312640]
International Journal of Environmental Research and Public Health Marathon Run-induced Changes in the Erythropoietin-Erythroferrone-Hepcidin Axis are Iron Dependent [Pubmed: 32316587]
VETERINARY MICROBIOLOGY Upregulation of HMGB1 Secretion in Lungs of Pigs Infected by Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus [Pubmed: 33221069]
Lab Invest Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis [Pubmed: 32709888]
American Journal of Translational Research Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and … [Pubmed: 32509190]
STEM CELL REVIEWS AND REPORTS A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … [Pubmed: 32406006]
Translational Stroke Research Glycyrrhizin Prevents Hemorrhagic Transformation and Improves Neurological Outcome in Ischemic Stroke with Delayed Thrombolysis Through Targeting?… []
stem cells international Hypoxic Wharton's Jelly Stem Cell Conditioned Medium Induces Immunogenic Cell Death in Lymphoma Cells []
Frontiers in Cell and Developmental Biology Aberrant Expression of High Mobility Group Box Protein 1 in the Idiopathic Inflammatory Myopathies []
Naja sumatrana venom cytotoxin, sumaCTX exhibits concentration-dependent cytotoxicity via caspase-activated mitochondrial-mediated apoptosis without?… []
Journal of Burn Care & Research Umbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in rats [33313794]
Cell Death & Disease DAMPs released by pyroptotic cells as major contributors and therapeutic targets for CAR-T-related toxicities [33504767]
Cells Insights into P-Glycoprotein Inhibitors: New Inducers of Immunogenic Cell Death [32331368]
Infect Immun A non-lethal murine flame burn model leads to a transient reduction in host defenses and enhanced susceptibility to lethal Pseudomonas aeruginosa infection [34152806]
Inhaled [D-Ala2]-Dynorphin 1-6 Prevents Hyperacetylation and Release of High Mobility Group Box 1 in a Mouse Model of Acute Lung Injury [34616852]
Mol Cell Biochem Autophagy-associated HMGB-1 as a novel potential circulating non-invasive diagnostic marker for detection of Urothelial Carcinoma of Bladder [34796446]
World J Gastroenterol Calycosin attenuates severe acute pancreatitis-associated acute lung injury by curtailing high mobility group box 1-induced inflammation [34908806]
Endocr J Clinical significance of high mobility group box 1/toll-like receptor 4 in obese diabetic patients [34657898]
Research in Veterinary Science Choline or CDP-choline restores hypotension and improves myocardial and respiratory functions in dogs with experimentally–Induced endotoxic shock [34715589]
Sci Rep Intracellular translocation of HMGB1 is important for Zika virus replication in Huh7 cells [Pubmed:35058496]
ORL J Otorhinolaryngol Relat Spec High Mobility Group Box-1 Protein and Interleukin 33 Expression in Allergic Rhinitis [Pubmed:34979505]
J Exp Clin Cancer Res SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma [Pubmed:35197103]
Am J Physiol Gastrointest Liver Physiol Abnormal intestinal milieu in post-traumatic stress disorder is not impacted by treatment that improves symptoms [Pubmed:35638693]
Journal of the South African Veterinary Association Circulating markers of endothelial activation in canine parvoviral enteritis []
Reseach square The antiviral action of resveratrol against Zika virus through the regulation of interferon responses by HMGB1 []
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