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組氨酸豐富糖蛋白(HRG)活性蛋白

Active Histidine Rich Glycoprotein (HRG)

HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG

血液病學(xué)

編號APC534Hu61
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
緩沖液成份PBS, pH7.4, containing 5% Trehalose.
性狀凍干粉
純度> 97%
等電點(diǎn)7.0
應(yīng)用Cell?culture;?Activity?Assays.
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規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

Gene sequencing

Figure. SDS-PAGE
WB圖

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Human histidine-rich glycoprotein (HRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525?aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. As HRG has the function of cell adhesion, we measure the activity of recombinant human HRG by the ability of the immobilized protein to support the adhesion of con-A activated MOLT4 human acute lymphoblastic leukemia cells. When 1 x 105?cells/well with 7.5 ug/ml conA are added to recombinat human HRG coated plates (0.1 μg/mL with 100 μL/well), >70% cells will adhere after 2?hour incubation at 37 ℃. The adhesion of MOLT-4 after 2?hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈(zèng)品

聚丙烯酰胺凝膠電泳(PAGE)實(shí)驗(yàn)服務(wù)

增值服務(wù)

BCA蛋白定量檢測試劑盒
 蛋白分子量MARKER
單克隆抗體定制服務(wù)
多克隆抗體定制服務(wù)
蛋白活性測定實(shí)驗(yàn)服務(wù)
電泳遷移率變動(dòng)分析(EMSA)實(shí)驗(yàn)服務(wù)
緩沖液
 慢病毒包裝實(shí)驗(yàn)服務(wù)
腺病毒包裝實(shí)驗(yàn)服務(wù)
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
棘突蛋白受體結(jié)合結(jié)構(gòu)域
 蛋白G
蛋白 A

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPC534Hu01	組氨酸豐富糖蛋白(HRG)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APC534Hu61	組氨酸豐富糖蛋白(HRG)活性蛋白	Cell?culture;?Activity?Assays.
EPC534Hu61	組氨酸豐富糖蛋白(HRG)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAC534Hu01	組氨酸豐富糖蛋白(HRG)多克隆抗體	WB; IHC; ICC; IP.
LAC534Hu71	組氨酸豐富糖蛋白(HRG)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAC534Hu24	組氨酸豐富糖蛋白(HRG)單克隆抗體	WB; IHC; ICC; IP.
MAC534Hu22	組氨酸豐富糖蛋白(HRG)單克隆抗體	WB; IHC; ICC; IP.
MAC534Hu21	組氨酸豐富糖蛋白(HRG)單克隆抗體	WB; IHC; ICC; IP.
MAC534Hu23	組氨酸豐富糖蛋白(HRG)單克隆抗體	WB; IHC; ICC; IP.
MAC534Hu26	組氨酸豐富糖蛋白(HRG)單克隆抗體	WB; IHC; ICC; IP.
SEC534Hu	組氨酸豐富糖蛋白(HRG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC534Hu	組氨酸豐富糖蛋白(HRG)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志	參考文獻(xiàn)
J. Proteome Res.	Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS [ACS: pr300330z]
Medical Oncology	Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancer [Springer: Source]
Int J Cancer	The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine‐rich glycoprotein [PubMed: 25363753]
Nucleic Acid Therapeutics	Pharmacokinetic and Pharmacodynamic Investigations of ION-353382, a Model Antisense Oligonucleotide: Using Alpha-2-Macroglobulin and Murinoglobulin Double-Knockout Mice [Pubmed:27031383]
United States Patent Application	Compositions and Methods for Diagnosing Lung Cancer [y2016:0077095.html]
Hypertension?Research	Combination of serum histidine-rich glycoprotein and uterine artery Doppler to predict preeclampsia [Pubmed:29416108]
journal of histochemistry & cytochemistry	Ultrastructural Localization of Histidine-rich Glycoprotein in Skeletal Muscle Fibers: Colocalization With AMP Deaminase [Pubmed: 31880188]
DOKLADY BIOCHEMISTRY AND BIOPHYSICS	Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … [Pubmed: 32342301]

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