干擾素α(IFNa)活性蛋白
Active Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- 編號APA033Mu61
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點9.1
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 2160 ¥ 5400 ¥ 10800 ¥ 32400 ¥ 81000
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產品包裝(模擬)
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產品包裝(模擬)
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Gene sequencing
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant IFNa, Mouse.
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
The Interferon-alpha?(IFNa) proteins belong to type I interferons (IFNs) which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins produced by leukocytes are also known as leukocyte interferon. They are mainly involved in innate immune response against viral infection. Besides, Interferon-alpha?13 (IFNA13) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IFNa and recombinant mouse IFNA13. Briefly, IFNA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNA13-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IFNapAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNA13 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| EPA033Mu61 | 干擾素α(IFNa)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA033Mu01 | 干擾素α(IFNa)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA033Mu02 | 干擾素α(IFNa)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA033Mu61 | 干擾素α(IFNa)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA033Mu01 | 干擾素α(IFNa)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA033Mu06 | 干擾素α(IFNa)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA033Mu01 | 干擾素α(IFNa)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA033Mu02 | 干擾素α(IFNa)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA033Mu71 | 干擾素α(IFNa)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA033Mu21 | 干擾素α(IFNa)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA033Mu22 | 干擾素α(IFNa)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA033Mu72 | 干擾素α(IFNa)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA033Mu76 | 干擾素α(IFNa)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA033Mu | 干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA033Mu | 干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA033Mu | 干擾素α(IFNa)檢測試劑盒(酶聯免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA033Mu | 干擾素α(IFNa)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA033Mu | 干擾素α(IFNa)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA033Mu01 | 干擾素α(IFNa)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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