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白介素3受體α(IL3Ra)活性蛋白

Active Interleukin 3 Receptor Alpha (IL3Ra)

CD123; IL3R-A; IL3RY; IL3RAY; IL3R; IL3RAY; IL3RY; hIL-3Ra

  • 白介素3受體α(IL3Ra)活性蛋白 產(chǎn)品包裝(模擬)
  • 白介素3受體α(IL3Ra)活性蛋白 產(chǎn)品包裝(模擬)
  • APB505Hu61.jpg SDS-PAGE圖
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity human IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. Both the alpha and the beta subunits are members of the cytokine receptor superfamily. A binding ELISA assay was conducted to detect the interaction of recombinant human Interleukin 3 Receptor Alpha and recombinant human Interleukin 3. Briefly, IL3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL3Ra-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL3Ra and IL3 was shown in Figure 1.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB505Hu01 白介素3受體α(IL3Ra)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB505Hu02 白介素3受體α(IL3Ra)活性蛋白 Cell?culture;?Activity?Assays.
APB505Hu61 白介素3受體α(IL3Ra)活性蛋白 Cell?culture;?Activity?Assays.
EPB505Hu61 白介素3受體α(IL3Ra)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB505Hu02 白介素3受體α(IL3Ra)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB505Hu01 白介素3受體α(IL3Ra)多克隆抗體 WB; IHC
LAB505Hu71 白介素3受體α(IL3Ra)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAB505Hu21 白介素3受體α(IL3Ra)單克隆抗體 WB; IHC; ICC; IP.
MAB505Hu22 白介素3受體α(IL3Ra)單克隆抗體 WB; IHC; ICC; IP.
FAB505Hu02 抗白介素3受體α(IL3Ra)單克隆抗體 Flow cytometry.
FAB505Hu04 抗白介素3受體α(IL3Ra)單克隆抗體 FCM
FAB505Hu06 抗白介素3受體α(IL3Ra)單克隆抗體 FCM
FAB505Hu08 抗白介素3受體α(IL3Ra)單克隆抗體 FCM
SEB505Hu 白介素3受體α(IL3Ra)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB505Hu 白介素3受體α(IL3Ra)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
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