異檸檬酸脫氫酶2(IDH2)活性蛋白
Active Isocitrate Dehydrogenase 2, mitochondrial (IDH2)
IDH; IDP; ICD-M; IDHM; mNADP-IDH; Isocitrate dehydrogenase [NADP], mitochondrial; Oxalosuccinate decarboxylase
- 編號APH838Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點8.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Isocitrate Dehydrogenase 2 (IDH2) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP ?as the electron acceptor and the other using NAD . The protein encoded by this gene is the NADP dependent isocitrate dehydrogenase found in the mitochondrial. The activity of recombinant human IDH2 was measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The reaction was performed in 25 mM Tris, 0.5 mM MnCl2, 5 mM DTT, pH 7.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of IDH2 (diluted by Assay Buffer) to 50 μL of substrate mixture consisted of 1 mM NADP and 2 mM isocitric acid. The final well serves as a negative control with no IDH2, replaced with 50μl assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant human IDH2 is >200 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APH838Hu01 | 異檸檬酸脫氫酶2(IDH2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPH838Hu01 | 異檸檬酸脫氫酶2(IDH2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH838Hu01 | 異檸檬酸脫氫酶2(IDH2)多克隆抗體 | WB,IHC |
| MAH838Hu21 | 異檸檬酸脫氫酶2(IDH2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEH838Hu | 異檸檬酸脫氫酶2(IDH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMH838Hu | 異檸檬酸脫氫酶2(IDH2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Molecular Oncology | IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancer [Pubmed:29465809] |
