Lemur酪氨酸激酶3(LMTK3)活性蛋白
Active Lemur Tyrosine Kinase 3 (LMTK3)
LMR3; TYKLM3; Serine/threonine-protein kinase LMTK3
- 編號(hào)APE375Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)6.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant LMTK3, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM?prior to the addition of various concentrations of LMTK3. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.
Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of LMTK3 was shown in Figure 2. It was obvious that LMTK3 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is approximately 6.14~50ng/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPE375Hu01 | Lemur酪氨酸激酶3(LMTK3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APE375Hu01 | Lemur酪氨酸激酶3(LMTK3)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAE375Hu01 | Lemur酪氨酸激酶3(LMTK3)多克隆抗體 | WB; IHC; ICC; IP. |
| MAE375Hu21 | Lemur酪氨酸激酶3(LMTK3)單克隆抗體 | WB; IHC; ICC; IP. |
| SEE375Hu | Lemur酪氨酸激酶3(LMTK3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME375Hu | Lemur酪氨酸激酶3(LMTK3)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Medical Oncology | Serum lemur tyrosine kinase 3 expression in colorectal cancer patients predicts cancer progression and prognosis [Pubmed:24174317] |
| Int J Clin Exp Pathol | Serum lemur tyrosine kinase-3: a novel biomarker for screening primary non-small cell lung cancer and predicting cancer progression [Pubmed:4348877] |
| Int J Clin Exp Med | Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … [PubMed: 26309506] |
