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脂多糖結合蛋白(LBP)活性蛋白

Active Lipopolysaccharide Binding Protein (LBP)

LPS-Binding Protein

  • 脂多糖結合蛋白(LBP)活性蛋白 產品包裝(模擬)
  • 脂多糖結合蛋白(LBP)活性蛋白 產品包裝(模擬)
  • APB406Mu02.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4.This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). It has been reported that LBP can enhance LPS-stimulated IL-8 secretion by THP-1 cells. To test the bioactivity of LBP, THP-1 cells were seeded into 24-well plate at a density of 1x106 cells/mL including 1ug/mL LPS, and treated with certain concentrations (0.625ug/mL-20ug/mL) of LBP for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of THP-1 cells increased significantly after stimulated with LBP have shown in Figure1.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當?shù)臈l件下存儲,損失率低于5%。

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
APB406Mu01 脂多糖結合蛋白(LBP)活性蛋白 Cell?culture;?Activity?Assays.
RPB406Mu02 脂多糖結合蛋白(LBP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB406Mu01 脂多糖結合蛋白(LBP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB406Mu02 脂多糖結合蛋白(LBP)活性蛋白 Cell?culture;?Activity?Assays.
PAB406Mu01 脂多糖結合蛋白(LBP)多克隆抗體 WB; IHC; ICC; IP.
PAB406Mu02 脂多糖結合蛋白(LBP)多克隆抗體 WB; IHC; ICC; IP.
SEB406Mu 脂多糖結合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB406Mu 脂多糖結合蛋白(LBP)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

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