巨噬細(xì)胞炎性蛋白1α(MIP1a)活性蛋白
Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)
CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein
- 編號APA092Mu61
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點4.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis?assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers and various concentrations of MIP-1a was added in lower chamber with a polycarbonate filter (8 um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted using Image J. The migrated THP1 cells in low chamber at low magnification (×100) was shown in Figure 1. The number of migrated THP-1 cells was shown in Figure 2. The optimum chemotaxis of MIP-1a occurs at 1 ug/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA092Mu01 | 巨噬細(xì)胞炎性蛋白1α(MIP1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA092Mu61 | 巨噬細(xì)胞炎性蛋白1α(MIP1a)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA092Mu02 | 巨噬細(xì)胞炎性蛋白1α(MIP1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA092Mu61 | 巨噬細(xì)胞炎性蛋白1α(MIP1a)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA092Mu01 | 巨噬細(xì)胞炎性蛋白1α(MIP1a)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA092Mu02 | 巨噬細(xì)胞炎性蛋白1α(MIP1a)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA092Mu71 | 巨噬細(xì)胞炎性蛋白1α(MIP1a)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MEA092Mu | 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA092Mu | 巨噬細(xì)胞炎性蛋白1α(MIP1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA092Mu | 巨噬細(xì)胞炎性蛋白1α(MIP1a)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| CGA092Mu01 | 巨噬細(xì)胞炎性蛋白1α(MIP1a)cDNA | PCR、蛋白表達(dá)等 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Inhalation Toxicology | Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans [Pubmed: 24044680] |
| Scientific Reports | The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37Ra [Pubmed:26912204] |
| ActaMedicaMediterranea | CIRCULATING MACROPHAGE INFLAMMATORY PROTEIN-1 ALPHA (MIP1-Α) AS A POTENTIAL BIOMARKER FOR THE DIAGNOSIS OF PATIENTS WITH … [57e97ac908aeb34bc08fd41a.pdf] |
| National Natural Scientific | Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure [pubmed:29121396] |
| Pharmacological Reports | Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorder [10.1016/j.pharep.2017.08.008] |
| NPG?Asia?Materials | Molybdenum disulfide/graphene oxide nanocomposites show favorable lung targeting and enhanced drug loading/tumor-killing efficacy with improved?… [Articles:am2017225] |
| Journal of Immunology?Research | Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat [Pubmed:29682587] |
| Journal of Immunology?Research | Cathelicidin LL-37 affects surface and intracellular Toll-like receptor expression in tissue mast cells [Pubmed:29670923] |
| Pharmacological Reports | Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without?… [Pubmed:29339257] |
| Journal?of?Food?Science | CQPC06 Activity Prevents Dextran Sulfate Sodium‐Induced Colitis by Regulating the IL‐8 Pathway [Doi: 10.1111/1750-3841.14346] |
| Biochemical and Biophysical Research Communications | The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusion [Pubmed: 30558792] |
| LIFE SCIENCES | Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stress [Pubmed: 33227274] |
| ACTA PHARMACOLOGICA SINICA | α-Galactosylceramide and its analog OCH differentially affect the pathogenesis of ISO-induced cardiac injury in mice [Pubmed: 32973325] |
| Life Sci | PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape [Pubmed: 32971102] |
| Nature Communications | A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy [Pubmed: 32332752] |
| Neoplasia | PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1 [34325342] |
