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巨噬細胞炎性蛋白1β(MIP1b)活性蛋白

Active Macrophage Inflammatory Protein 1 Beta (MIP1b)

CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b

  • 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 產(chǎn)品包裝(模擬)
  • 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 產(chǎn)品包裝(模擬)
  • APA093Hu02.png Figure. SDS-PAGE
  • 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 Sample: Recombinant MIP1b, Human; <br/>Antibody: Rabbit Anti-Human MIP1b Ab (PAA093Hu02)<br/>Figure. Western Blot
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實驗

Macrophage inflammatory protein-1β (MIP-1β), also known as CCL4 is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. MIP-1β is a kind of chemotactic cytokine. Thus, chemotaxis?assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of MIP-1β on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100uL cell suspension, 5×105 cells/mL in RPMI 1640 with FBS free) and recombinant human MIP-1β (0.0001ng/mL, 0.001ng/mL, 0.1ng/mL, 1ng/mL, 10ng/mL, 100ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the <br/>filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×200) randomly (five fields for each filter). Result shows recombinant human MIP-1β is able to induce migration of Jurkat cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×200). Statistical results?were shown in Figure 2. The optimum chemotaxis of MIP-1β occurs at 0.1~10pg/mL.<br/>(A)Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 with 0.01ng/mL MIP-1β was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 2h; <br/>(B)Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 without MIP-1β was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 2h.<br/>Figure. The chemotactic effect of recombinant human MIP-1β on Jurkat cells.

Figure. The chemotactic effect of recombinant human MIP1-βon Jurkat cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA093Hu01 巨噬細胞炎性蛋白1β(MIP1b)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA093Hu61 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 Cell?culture;?Activity?Assays.
APA093Hu62 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 Cell?culture;?Activity?Assays.
APA093Hu01 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 Cell?culture;?Activity?Assays.
EPA093Hu61 巨噬細胞炎性蛋白1β(MIP1b)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA093Hu62 巨噬細胞炎性蛋白1β(MIP1b)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA093Hu02 巨噬細胞炎性蛋白1β(MIP1b)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA093Hu02 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 Cell?culture;?Activity?Assays.
PAA093Hu01 巨噬細胞炎性蛋白1β(MIP1b)多克隆抗體 WB; IHC; ICC; IP.
MAA093Hu22 巨噬細胞炎性蛋白1β(MIP1b)單克隆抗體 WB; IHC; ICC; IP.
SEA093Hu 巨噬細胞炎性蛋白1β(MIP1b)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA093Hu 巨噬細胞炎性蛋白1β(MIP1b)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Inhalation Toxicology Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans [Pubmed: 24044680]
PLoS One. Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats. [Pubmed: 24647044]
PLOS ONE Megakaryocytic Leukemia 1 (MKL1) Regulates Hypoxia Induced Pulmonary Hypertension in Rats [Pubmed:Pmc3960100]
Cell Physiol Biochem MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1 [PubMed: 26159489]
Am J Alzheimers Dis Other Demen. Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease [Pubmed:26993489]
Pharmacological Reports The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder [10.1016:j.pharep.2018.06.001]
Metabolic Brain Disease Neuroprotective effects of Bacopa monnieri in Parkinson's disease model [Pubmed: 31834548]
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