巨噬細胞炎性蛋白1β(MIP1b)活性蛋白
Active Macrophage Inflammatory Protein 1 Beta (MIP1b)
CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b
- 編號APA093Hu02
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 97%
- 等電點4.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1622 ¥ 4056 ¥ 8112 ¥ 24336 ¥ 60840
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活性實驗
Macrophage inflammatory protein-1β (MIP-1β), also known as CCL4 is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. MIP-1β is a kind of chemotactic cytokine. Thus, chemotaxis?assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of MIP-1β on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100uL cell suspension, 5×105 cells/mL in RPMI 1640 with FBS free) and recombinant human MIP-1β (0.0001ng/mL, 0.001ng/mL, 0.1ng/mL, 1ng/mL, 10ng/mL, 100ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the <br/>filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×200) randomly (five fields for each filter). Result shows recombinant human MIP-1β is able to induce migration of Jurkat cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×200). Statistical results?were shown in Figure 2. The optimum chemotaxis of MIP-1β occurs at 0.1~10pg/mL.<br/>(A)Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 with 0.01ng/mL MIP-1β was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 2h; <br/>(B)Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 without MIP-1β was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 2h.<br/>Figure. The chemotactic effect of recombinant human MIP-1β on Jurkat cells.
Figure. The chemotactic effect of recombinant human MIP1-βon Jurkat cells.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA093Hu01 | 巨噬細胞炎性蛋白1β(MIP1b)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA093Hu61 | 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 | Cell?culture;?Activity?Assays. |
APA093Hu62 | 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 | Cell?culture;?Activity?Assays. |
APA093Hu01 | 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 | Cell?culture;?Activity?Assays. |
EPA093Hu61 | 巨噬細胞炎性蛋白1β(MIP1b)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA093Hu62 | 巨噬細胞炎性蛋白1β(MIP1b)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA093Hu02 | 巨噬細胞炎性蛋白1β(MIP1b)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA093Hu02 | 巨噬細胞炎性蛋白1β(MIP1b)活性蛋白 | Cell?culture;?Activity?Assays. |
PAA093Hu01 | 巨噬細胞炎性蛋白1β(MIP1b)多克隆抗體 | WB; IHC; ICC; IP. |
MAA093Hu22 | 巨噬細胞炎性蛋白1β(MIP1b)單克隆抗體 | WB; IHC; ICC; IP. |
SEA093Hu | 巨噬細胞炎性蛋白1β(MIP1b)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA093Hu | 巨噬細胞炎性蛋白1β(MIP1b)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
Inhalation Toxicology | Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans [Pubmed: 24044680] |
PLoS One. | Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats. [Pubmed: 24647044] |
PLOS ONE | Megakaryocytic Leukemia 1 (MKL1) Regulates Hypoxia Induced Pulmonary Hypertension in Rats [Pubmed:Pmc3960100] |
Cell Physiol Biochem | MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1 [PubMed: 26159489] |
Am J Alzheimers Dis Other Demen. | Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease [Pubmed:26993489] |
Pharmacological Reports | The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder [10.1016:j.pharep.2018.06.001] |
Metabolic Brain Disease | Neuroprotective effects of Bacopa monnieri in Parkinson's disease model [Pubmed: 31834548] |