神經(jīng)生長(zhǎng)因子(NGF)活性蛋白
Active Nerve Growth Factor (NGF)
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
- 編號(hào)APA105Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱(chēng)，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)9.4
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1488 ¥ 3720 ¥ 7440 ¥ 22320 ¥ 55800
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Nerve Growth Factor?(NGF) is the archetypal?neurotrophin?of a family of?polypeptides?.It has long occupied a critical role in developmental and adult neurobiology for its many important regulatory functions on the survival, growth and differentiation of nerve cells in the peripheral and central nervous system. The Caspase 3 (CASP3) is high affinity receptor for Nerve Growth Factor，thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse NGF and recombinant human CASP3. Briefly, NGF was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CASP3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse NGF and recombinant human CASP3 was shown in Figure 1, the EC50?for this effect is 0.86 ug/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA105Mu01 | 神經(jīng)生長(zhǎng)因子(NGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA105Mu01 | 神經(jīng)生長(zhǎng)因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA105Mu02 | 神經(jīng)生長(zhǎng)因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA105Mu03 | 神經(jīng)生長(zhǎng)因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA105Mu01 | 神經(jīng)生長(zhǎng)因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA105Mu02 | 神經(jīng)生長(zhǎng)因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
| MEA105Mu | 神經(jīng)生長(zhǎng)因子(NGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA105Mu | 神經(jīng)生長(zhǎng)因子(NGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA105Mu | 神經(jīng)生長(zhǎng)因子(NGF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Carbohydrate Polymers | Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration [Pubmed:35550777] |
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